畜牧与兽医2025，Vol.57Issue(6)：20-25,6.
唾液乳杆菌的分离鉴定及其对产蛋后期蛋鸡产蛋率、蛋品质和血清生化指标的影响
Isolation and identification of Lactobacillus salivarius and its effects on laying rate,egg quality and serum biochemical indices in late-phase laying hens
摘要
Abstract
In this study,lactic acid bacteria were isolated and identified from the gut of healthy white feather broilers.Bacterial isolation and culture techniques,16S rRNA sequencing,the Oxford Cup diffusion method were used to detect bacteriostatic ability,pH and bile salt tolerance in the hens.Finally,strains were screened by means of the lactic acid production ability of the bids.A total of two hundred and sev-enty 500 days old green shell laying hens with similar weight and laying rate were used and randomly divided into 3 groups,including the control group,the low dose Lactobacillus salivary group and the high dose lactobacillus salivary group.Then,the effects of Lactobacillus sali-vary on the laying rate,egg quality and serum biochemical indexes of the laying hens in late laying period were investigated.The experiment lasted for 6 weeks.The results showed that the isolated bacteria were Gram-positive bacteria with strong acid-producing ability,and they were named Lactobacillus salivarius JQ07,based on the identification results of 16S rRNA.The fermentation supernatant of the bacteria could inhibit the growth of Escherichia coli,Salmonella and Staphylococcus aureus,and the bacteria had certain characteristics of acid and bile salt resistance.The animal test results showed that,compared with the control group,the laying rate of the high dose Lactobacillus salivary group was significantly increased(P＜0.05).The addition of Lactobacillus salivarius improved the egg quality,including Haugh unit,eggshell thickness and strength.In conclusion,Lactobacillus salivary JQ07 derived from the gut of healthy white feather broilers improved the laying rate and egg quality of late-laying period hens by enhancing their intestinal digestion and absorption function.The strain contributed to the production performance of late-phase laying hens,and could be used as a candidate strain of probiotics.关键词
蛋鸡/唾液乳杆菌/产蛋率/生化指标Key words
laying hens/Lactobacillus salivarius/laying rate/biochemical indices分类
畜牧业引用本文复制引用
陈大伟,刘茵茵,马丽娜,唐修君,葛庆联,陆俊贤,丁红梅,高玉时..唾液乳杆菌的分离鉴定及其对产蛋后期蛋鸡产蛋率、蛋品质和血清生化指标的影响[J].畜牧与兽医,2025,57(6):20-25,6.基金项目
扬州市社会发展项目(YZ2023078) （YZ2023078）
扬州市现代农业项目(YZ2023057) （YZ2023057）
江西省技术创新引导类计划项目(20212BDH80013) （20212BDH80013）
江苏现代农业(肉鸡)产业技术体系项目(JATS[2023]363) （肉鸡）
