畜牧与兽医2025，Vol.57Issue(6)：31-37,7.
lncRNA CUFF.253988.1在T-2毒素诱导猪卵巢颗粒细胞损伤中的作用
Effect of lncRNA CUFF.253988.1 on T-2 toxin-induced ovarian granulosa cell injury in porcine
摘要
Abstract
This study aimed to explore the effect of lncRNA CUFF.253988.1 on T-2 toxin-induced ovarian granulosa cell injury in por-cine.Here,primary porcine ovarian granulosa cells were isolated and cultured,and were then identified by fluorescence-labeled FSHR anti-body.The RT-qPCR was employed to assess the impact of T-2 toxin on ovarian granulosa cells and their mitochondrial lncRNA CUFF.253988.1 transcription levels.Additionally,the RNA fluorescence in situ hybridization technology was utilized to examine the effect of T-2 toxin on the cellular localization of lncRNA CUFF.253988.1.Flow cytometry was employed to analyze the effects of T-2 toxin on reac-tive oxygen species(ROS),mitochondrial membrane potential,and apoptosis of the ovarian granulosa cells.Furthermore,transmission elec-tron microscopy was used to observe the impact of T-2 toxin on cell ultrastructure.Antisense oligonucleotide(ASO)was specifically em-ployed to silence lncRNA CUFF.253988.1,and subsequent changes in relevant indicators were monitored.The results showed that T-2 toxin notably boosted the levels of lncRNA CUFF.253988.1 in both cells and mitochondria(P＜0.01),leading to enhanced concentration of lncRNA CUFF.253988.1 within the mitochondria.Upon silencing lncRNA CUFF.253988.1,the T-2 toxin-induced structural damage to the mitochondria was mitigated,resulting in a marked reduction in cellular ROS generation(P＜0.01),restoration of mitochondrial mem-brane potential(P＜0.01),and inhibition of cellular apoptosis(P＜0.01).These results indicated that lncRNA CUFF.253988.1 played an important regulatory role in T-2 toxin-induced porcine ovarian granulosa cell injury.关键词
lncRNA CUFF.253988.1/猪卵巢颗粒细胞/线粒体损伤/氧化应激/细胞凋亡Key words
lncRNA CUFF.253988.1/porcine ovarian granulosa cells/mitochondrial injury/oxidative stress/cell apoptosis分类
畜牧业引用本文复制引用
范慧,吴悠,肖波,杨成林,刘水平,邬静..lncRNA CUFF.253988.1在T-2毒素诱导猪卵巢颗粒细胞损伤中的作用[J].畜牧与兽医,2025,57(6):31-37,7.基金项目
云南省重大科技专项计划项目(202202AE090032) （202202AE090032）
