畜牧与兽医2025，Vol.57Issue(6)：38-43,6.
一株羊源高产纤维素酶枯草芽胞杆菌的分离鉴定及生物学特性研究
Isolation,identification and biological characteristics of a high-yield cellulase Bacillus subtilis
摘要
Abstract
This study aimed to screen potential high yield cellulase probiotics and to explore its probiotic effect to be used in production practice.Carboxymethyl cellulose plates were used to screen cellulose strains from the intestinal contents of sheep,in order to determine the cellulase activity and to select the target strain.Then,morphological analysis,biochemical tests and sequence analysis of the 16S rDNA gene were used to identify the target strain species.Finally,it's the growth curve,protease and α-amylase production capacity,pathogen inhibi-tion capacity,acid and bile salt resistance capacity,drug sensitivity,and host safety of the strain.The results showed that eight cellulase-producing strains were selected from the samples.The most cellulose-producing strain ECL1.2 was selected according to the ratio of transpar-ent circle(H)and colony diameter(C)and cellulase activity.The carboxymethylcellulase activity was 18.61 U/mL,which was identified as Bacillus subtilis.ECL1.2 entered the logarithmic growth phase after 16 h of culture and reached a plateau at 24 h of culture.Its protease and α-amylase viability were 2.08 U/mL and 19.7 U/mL,respectively,and it inhibited the growth of Escherichia coli,Salmonella enteriti-dis and Staphylococcus aureus.Being in pH 2-7 culture for 4 h and in the culture with bile salt at concentration of 0.1％-0.3％culture for 8 h,the strain had a survival rate over than 60％,with a little acid resistance and bile salt resistance.ECL 1.2 had low sensitivity to lin-comycin,intermediate sensitivity to gentamicin,and high sensitive to penicillin,cefazolin,clarithromycin,norfloxacin,vancomycin,and ri-fampin.It showed safety for mouse organs and intestines,and was helpful for their daily weight gain and food intake.In conclusion,Bacillus subtilis ECL 1.2 could be used as a potential probiotic fermentation strain.关键词
微生物发酵/纤维素酶/分离鉴定/生物学特性/益生菌Key words
microbial fermentation/cellulase/isolation and identification/biological characteristics/probiotics分类
畜牧业引用本文复制引用
吴非凡,杨明凡,王晶,常伟辰,孔亮,黎露露,徐曾浩,毛赛,张红英,袁淑萍..一株羊源高产纤维素酶枯草芽胞杆菌的分离鉴定及生物学特性研究[J].畜牧与兽医,2025,57(6):38-43,6.基金项目
河南省重点研发项目(221111111300) （221111111300）
河南省重点研发专项(231111111600) （231111111600）
