畜牧与兽医2025，Vol.57Issue(6)：52-60,9.
江苏与宁夏部分地区鸡源金黄色葡萄球菌的耐药性调查
Investigation of antimicrobial resistance of Staphylococcus aureus isolated from chickens in parts of Jiangsu and Ningxia
摘要
Abstract
In this study,we investigated the antimicrobial resistance of Staphylococcus aureus isolated from laying hens and broilers in some farms in Jiangsu Province and the Ningxia Hui Autonomous Region.Staphylococcus aureus was isolated from the selected chickens and were identified by enrichment,color culture of Staphylococcus aureus,and PCR identification.Then,the broth microdilution method was used to detect the minimum inhibitory concentrations(MICs)of 18 antibacterial drugs against the isolated strains,and PCR was used to detect 17 common resistance genes.The results showed that 64 strains of Staphylococcus aureus were isolated from 760 cloacal swabs,cecum,and fecal samples;the strains included 42 from Jiangsu and 22 strains from Ningxia with isolation rates of 8.24％and 8.80％,respectively.There was no significant difference in the detection rate of Staphylococcus aureus between the chickens in the two regions.The drug resistance rates showed that the isolated strains were highly resistant to penicillin(61/64),ogmentin(60/64),erythromycin(58/64),clindamycin(59/64),enrofloxacin(56/64),ofloxacin(58/64),trimethoprim-sulfamethoxazole(59/64),florfenicol(60/64),telmicocin(52/64),gen-tamicin(56/64)and tetracycline(59/64),and the resistance rates were over 80％for all.The strains were sensitive to cefoxitin,sulfame-thoxazole and oxacillin,and their resistance rates were as low as 3.31％,6.25％and 7.81％,respectively.However,ceftiofur,vancomycin and linezolid resistant strains were not isolated.In general,the multi-antimicrobial resistance rate of the isolated strains was as high as 93.75％,including 90.48％in Jiangsu and 100％in Ningxia.There were 23 types of antimicrobial resistance profiles,and PEN-ERY-CLI-ENR-OFL-SXT-TET-FFC-TIL-GEN accounted for the highest proportion of the antimicrobial resistance phenotypes,with 21 strains.A to-tal of 13 resistance genes were detected,among which the detection rate of fluoroquinolone resistance gene grlA was 96.88％(62/64),and the detection rate of ciprofloxacin resistance gene norA was 95.31％(61/64).The results of this study indicated that the antimicrobial resist-ance rate of Staphylococcus aureus isolated from some chickens in Ningxia was generally higher than that of Staphylococcus aureus from some chickens in Jiangsu,and the multi-antimicrobial resistance was also serious.It was suggested that antimicrobial agents should be used rea-sonably and cautiously based on the drug sensitivity results,and the routine monitoring of bacterial resistance should be strengthened in all aspects of production.关键词
金黄色葡萄球菌/耐药性/基因型/江苏省/宁夏回族自治区Key words
Staphylococcus aureus/antimicrobial resistance/genotype/Jiangsu Province/Ningxia Hui Autonomous Region分类
畜牧业引用本文复制引用
赵文斌,赵佳琪,刘晓,代兴杨,黄金虎,王丽平,王晓明..江苏与宁夏部分地区鸡源金黄色葡萄球菌的耐药性调查[J].畜牧与兽医,2025,57(6):52-60,9.基金项目
国家重点研发计划项目(2022YFD1800400) （2022YFD1800400）
