畜牧与兽医2025，Vol.57Issue(6)：61-68,8.
基于RNA-seq探究光周期对扬州鹅睾丸发育的影响机制
Effect analysis of photoperiod on testis development of Yangzhou geese based on RNA-seq technology
摘要
Abstract
This study aimed to explore the mechanism of the effect of photoperiod on the development of goose testis.The experimental sub-jects were Yangzhou geese(735 days of age,male to female ratio of 1∶5),raised in fully enclosed artificial sheds with controlled light and temperature(1 500 geese per shed).During the feeding period,the light program was short light(L∶D=6.5 h∶17.5 h)feeding for 60 days,followed by extended light(L∶D=11.5 h∶12.5 h)feeding.The blood samples were collected from the geese on the 38th day of short light(the short light group)and the 73rd day of long light(the long light group),and serum was separated to detect relevant physio-logical and biochemical indicators and hormone levels.The left testicular tissue samples were used for transcriptome sequencing(RNA seq)analysis;and differentially expressed genes(DEGs)were screened between the short light and long light groups.The results showed that the testis volume was increased in the long light group,compared with that in the short light group,together with a large number of mature sperm in the seminiferous,increased seminiferous tubule diameter,epithelial height,and lumen diameter(P＜0.05).The levels of blood testoster-one(T),follicle-stimulating hormone(FSH),and luteinizing hormone(LH)concentrations were significantly higher in the long light group than those in the short light group.The transcriptome sequencing results showed that 4 383 genes were significantly upregulated and 2 928 genes were downregulated in the long light group,compared with those in the short light group.The DEGs were mainly enriched in ECM-receptor interaction,the PI3K-AKT signaling pathway,Ribosome,and so on.It can be concluded that the PI3K-AKT pathway was activated by prolonged light,thereby promoting the development of goose testis.This experiment provided important evidence for further un-derstanding the regulatory mechanism of light on animal reproduction.关键词
扬州鹅/光照/RNA-seq/睾丸Key words
Yangzhou goose/light exposure/transcriptome/testis分类
农业科技引用本文复制引用
冯宇妍,陈晓静,朱欢喜,倪迎冬,刘杰..基于RNA-seq探究光周期对扬州鹅睾丸发育的影响机制[J].畜牧与兽医,2025,57(6):61-68,8.基金项目
江苏省农业科技自主创新项目(CX(24)1012) （CX(24）
江苏省种业振兴"揭榜挂帅"项目(JBGS(2021)111) （JBGS(2021）
