畜牧与兽医2025，Vol.57Issue(6)：69-76,8.
一株H3N8亚型犬流感病毒的分离鉴定及其对小鼠致病性研究
Isolation,identification,and pathogenicity in mice of an H3N8 subtype canine influenza virus
摘要
Abstract
To investigate the epidemiological characteristics and potential public health risks of canine influenza virus(CIV)in Guangzhou area of China,this study systematically examined pet dogs with influenza-like symptoms in Guangzhou City,Guangdong Province.Nasal swabs were collected from the dogs for virus isolation using MDCK(Madin-Darby Canine Kidney)cell culture,followed by identification of isolated strains via reverse transcription polymerase chain reaction(RT-PCR).The virus was intranasally inoculated into 4-week-old BALB/c mice,which were divided into the control group and the infection group.The same dose of DMEM and influenza A virus(IAV)ven-om was intranasally inoculated respectively.Temperature sensitivity experiments were also conducted to determine viral replication character-istics in vitro.The results demonstrated successful isolation of an H3N8 subtype canine influenza virus strain(designated as IAV-2022-GZ).The infected mice exhibited clinical manifestations including sluggish movement,ruffled fur,and weight loss,while the DMEM-treated con-trol group maintained normal weight gain.The viral loads in the lungs and trachea of the infected mice were higher than those in the controls,indicating efficient viral replication in the murine lungs and suggesting a potential pathogenic capacity.The temperature sensitivity assays re-vealed thermosensitive characteristics of this strain.In conclusion,this study elucidated key biological features of the H3N8 subtype CIV,providing critical reference data for risk assessment of cross-species transmission and for comprehensive prevention strategies against influenza viruses.关键词
流感病毒/病毒分离鉴定/H3N8/BALB/c小鼠Key words
influenza virus/virus isolation and identification/H3N8/BALB/c mice分类
畜牧业引用本文复制引用
李鑫鑫,翟晓凤,粟硕..一株H3N8亚型犬流感病毒的分离鉴定及其对小鼠致病性研究[J].畜牧与兽医,2025,57(6):69-76,8.基金项目
国家重点研发计划项目(2022YFC2604203) （2022YFC2604203）
