畜牧与兽医2025，Vol.57Issue(6)：105-113,9.
伪狂犬病病毒三基因缺失疫苗株、Bartha-K61株对绵羊的安全性及其对变异毒株攻毒的保护作用
Safety and the protective function of a vaccine strain with three-virulence gene deletion and Bartha-K61 strain against variant pseudorabies virus challenge in sheep
摘要
Abstract
The aim of this study was to evaluate the safety of the three-gene deletion vaccine strain in sheep and its protective effect against vPRV/XJ5 strain.In this study,the variant pseudorabies virus(vPRV)-XJ5 gI-/gE-/TK-gene deleted vaccine strain constructed in the laboratory based on the vPRV/XJ5 strain and the Bartha-K61 strain were used in different doses to immunize 6-month-old sheep.These sheep were then challenged with the vPRV/XJ5 strain.Next,the antibody level after the immunization,rectal temperature after the chal-lenge,clinical symptoms,viral shedding and viral load in tissue in each group were determined to evaluate both the safety and the protective effect of the vPRV-XJ5 gI-/gE-/TK-strain or the Bartha-K61 strain in sheep.The results showed that the sheep which were immunized with 1×105 TCID50 or 1×106 TCID50 Bartha-K61 and 1×106 TCID50 vPRV-XJ5 gI-/gE-/TK-gene-deleted vaccine had no adverse reaction and maintained weight gain within 2 weeks post-vaccination,suggesting either the Bartha-K61 vaccine and or the vPRV-XJ5 gI-/gE-/TK-gene-deleted vaccine experienced an equal safety in sheep.The weight gain of the sheep in the 1×105 TCID50 vPRV-XJ5 gI-/gE-/TK-vac-cine immunized group and the challenge control group decreased after the challenge.The viral shedding detection results of the rectal swabs and pharyngeal swabs showed that the viral shedding of the sheep in the 106 TCID50 vPRV-XJ5 gI-/gE-/TK-or Bartha-K61 vaccine immu-nized groups was lower than that of the animals in the 105 TCID50 vaccine immunized groups.For vPRV currently circulated in field,neither the 1×105 TCID50 Bartha-K61 vaccine nor the vPRV-XJ5 gI-/gE-/TK-gene-deleted vaccine provide clinical protection against the fatal challenge of vPRV in sheep.However,the 1×106 TCID50 Bartha-K61 vaccine or the vPRV-XJ5 gI-/gE-/TK-gene-deleted vaccine can provide partially clinical protection for sheep.关键词
伪狂犬病/基因缺失疫苗/Bartha-K61/绵羊/保护力Key words
pseudorabies/gene-deleted vaccine/Bartha-K61/sheep/protection分类
农业科技引用本文复制引用
高地,张永帅,李爽,王小波,高崧..伪狂犬病病毒三基因缺失疫苗株、Bartha-K61株对绵羊的安全性及其对变异毒株攻毒的保护作用[J].畜牧与兽医,2025,57(6):105-113,9.基金项目
江苏省重点研发计划项目(BE2020320) （BE2020320）
江苏高校优势学科建设工程项目(PAPD) （PAPD）
