云南师范大学学报（自然科学版）2025，Vol.45Issue(2)：27-36,10.DOI:10.7699/j.ynnu.ns-2025-018
云南省马铃薯褐斑病病原群体遗传结构分析
Genetic Structure Analysis of Potato Brown Spot Pathogen Populations in Yunnan Province
摘要
Abstract
This study aims to systematically analyze the genetic diversity and population structure of Alternaria alternata(the causal agent of potato brown spot)in Yunnan Province,China,to explore its distribution characteristics and evolutionary processes.Using techniques such as simple sequence re-peat(SSR)markers and Bayesian clustering analysis,we revealed the genetic variation and migration patterns of this pathogen across different geographic regions.The results showed that Alternaria al-ternata is the dominant species,with a population structure exhibiting high genetic diversity,indicating its widespread distribution in Yunnan.SSR analysis identified 42 distinct genotypes,with 54.8％ap-pearing only once,further confirming the pathogen's high genetic diversity.Additionally,cluster analy-sis based on a similarity coefficient of 0.45 divided the isolates into two groups,which were further subdivided into three subgroups at a similarity coefficient of 0.63,with Zhaotong isolates showing sig-nificant differences.UPGMA clustering results indicated that isolates from Dali and Lijiang formed one cluster,while Wenshan isolates exhibited higher genetic similarity,possibly due to potato seed tuber transportation.However,Zhaotong isolates formed an independent branch due to geographic isolation,showing significant genetic structure differences.The study concluded that the genetic structure of A.alternata is closely related to its geographic distribution,with geographic isolation being the primary factor for genetic differentiation.Additionally,potato seed tuber transportation and circulation acceler-ated gene exchange,influencing the dispersion and evolutionary processes of the pathogen.关键词
马铃薯褐斑病/链格孢菌/SSR分子标记/聚类分析/遗传多样性Key words
Potato brown spot/Alternaria alternata/SSR molecular marker/Cluster analysis/Genetic diversity分类
农业科学引用本文复制引用
张向东,陈洁,吴彦瑢,高李玉,梁丽燕,谭晨,唐唯..云南省马铃薯褐斑病病原群体遗传结构分析[J].云南师范大学学报（自然科学版）,2025,45(2):27-36,10.基金项目
云南省基础研究计划资助项目(202301AS070010). （202301AS070010）
