医药导报2025，Vol.44Issue(6)：928-933,6.DOI:10.3870/j.issn.1004-0781.2025.06.015
酸枣多糖低共熔溶剂提取工艺优化及体外降糖活性
Ultrasonic-assisted Deep Eutectic Solvent Extraction of Ziziphusjujube Polysaccharide and Its Hypoglycemic Activity
摘要
Abstract
Objective To optimize the extraction process of Ziziphusjujube polysaccharides and study their hypoglycemic effect in vitro.Methods Using deep eutectic solvent as the extraction solvent,the optimal extraction condition for Ziziphusjujube polysaccharides was selected using single factor experiments and Box Behnken experiments.The selected factors were water content,solid-liquid ratio,and ultrasound time.At the same time,the content of Ziziphusjujube polysaccharideswas determined,and the in vitro hypoglycemic effect of polysaccharides and the hypoglycemic ability of different parts of Ziziphusjujube were investigated.Results After Box-Behnken response surface optimization,the optimal extraction condition for Ziziphusjujube polysaccharides was as follows:after ultrasonic treatment for 41.68 minutes,solid-to liquid ratio of 1∶20,and the water content in the deep eutectic solvent was 19.96%,under these conditions,the Ziziphusjujube polysaccharide content reach(571.31±3.84)mg·g-1 α-amylase inhibition rate of Ziziphusjujube polysaccharides was 76.08%,the content of polysaccharide in whole fruit of Ziziphusjujube was the highest,while the pulp exhibited the strongest hypoglycemic activity.Conclusion The optimized process can providea areference for the development in industry and clinical application of Ziziphusjujube.关键词
酸枣多糖/Box-Behnken响应面法/低共熔溶剂Key words
Ziziphusjujube polysaccharide/Box-Behnken response surface methodology/Deep eutectic solvent分类
医药卫生引用本文复制引用
王艳艳,汪子皓,李奕萱,王红坤,孟永海,翟春梅..酸枣多糖低共熔溶剂提取工艺优化及体外降糖活性[J].医药导报,2025,44(6):928-933,6.基金项目
国家自然科学基金资助项目(82003974) （82003974）
黑龙江省青年科学基金资助项目(LH2022H086) （LH2022H086）
黑龙江省中医药科研项目(202301) （202301）
黑龙江中医药大学实验中心"寒龙地药'五味宁果饮'的研究与开发"课题(KY2022-11). （KY2022-11）
