医药导报2025，Vol.44Issue(6)：934-941,8.DOI:10.3870/j.issn.1004-0781.2025.06.016
响应面法优化低共熔溶剂提取毛连菜中有机酸的提取工艺
Optimization of Extraction Process for Organic Acids from Picris hieracioides L.using Deep Eutectic Solventy by Response Surface Methodology
摘要
Abstract
Objective To optimize the extraction process of chlorogenic acid and three other organic acids from Picris hieracioides L.with deep eutectic solvents using response surface methodology.Methods By comparing the extraction rate of organic acids in seven deep eutectic solvents,the best solvent combinations were identified,and the optimal extraction process of organic acids from Picris hieracioides L.was obtained by optimizing the liquid/feed ratio,extraction temperature,extraction time and other parameters using the response surface method on the basis of a one-way experiment.Results The deep eutectic solvent with 30%water content(choline chloride∶urea=1∶2)was effective in extracting organic acids from Picris hieracioides L.The optimal extraction process optimized by response surface method was:extraction temperature 30℃,extraction time 30 min,liquid-solid ratio 20∶1(mL·g-1),the extraction rate of organic acids under this condition was 1.092 9%.Conclusion The deep eutectic solvent optimized through response surface methodology is an efficient,green and eco-friendly extraction method.This study provides a theoretical foundation for the subsequent development and utilization of Picris hieracioides L..关键词
毛连菜/低共熔溶剂/绿原酸/异绿原酸A/异绿原酸B/异绿原酸C/响应面法Key words
Picris hieracioides L./Deep eutectic solvents/Chlorogenic acid/Isochlorogenic acid A/Isochlorogenic acid B/Isochlorogenic acid C/Response surface methodology分类
医药卫生引用本文复制引用
叶方,彭雯婧,叶旭雯,王雪芹,卢伟,郑涛,黄良永..响应面法优化低共熔溶剂提取毛连菜中有机酸的提取工艺[J].医药导报,2025,44(6):934-941,8.基金项目
2021年度十堰市市级引导性科研项目(21Y03) （21Y03）
湖北医药学院"十四五"省级优势特色学科群(生物与医药)2022年资助项目(2022BMXKQT3). （生物与医药）
