中国动物检疫2025，Vol.42Issue(5)：45-51,7.DOI:10.3969/j.issn.1005-944X.2025.05.008
圈养西太平洋斑海豹口腔菌群多样性分析
Analysis of Oral Microbiota Diversity in Captive Western Pacific Spotted Seals
王玉英 1杨翠华 1杨敏 1程培赛 1魏巍 1黄大鹏 1靳鹏 1李超2
作者信息
- 1. 青岛水族馆,山东 青岛 266031
- 2. 中国动物卫生与流行病学中心,农业农村部动物生物安全风险预警及防控重点实验室(南方),青岛市动物生物安全重点实验室,山东 青岛 266032
- 折叠
摘要
Abstract
In order to analyze the species composition and diversity distribution characteristics of oral microbiota in captive Western Pacific spotted seals,relevant samples were collected from such captive seals using disposable flocking swabs to extract total nucleic acids,and microbiota abundance and species were identified through high-throughput sequencing.The results showed that 418 genera,217 families,126 orders,53 classes and 28 phyla were covered by the microbiota contained in the samples;Bacteroidota(33.76%),Proteobacteria(28.07%),Fusobacteriota(22.59%),Firmicutes(8.35%),Actinobacteria(2.42%),Patescibacteria(2.22%)and Campilobacterota(1.00%)with high abundance accounted for over 98%of the total oral microbiome in the samples.858,910,806 and 892 species were respectively identified from the samples numbered Xin1,CB1,XB2 and DD2,368 of them were shared by 4 samples,accounting for approximately 10.6%of all detected species.In addition,216,330,204 and 238 species were unique to samples Xin1,CB1,XB2 and DD2,respectively.In conclusion,the oral microbiota in captive Western Pacific spotted seals was with high diversity,and there were certain common or specific characteristics in different seals under the same captive environment.A theoretical basis was provided for the diagnosis and treatment of oral diseases in captive spotted seals.关键词
高通量测序/西太平洋斑海豹/口腔菌群/多样性分析Key words
high-throughput sequencing/Western Pacific spotted seal/oral microbiota/diversity analysis分类
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王玉英,杨翠华,杨敏,程培赛,魏巍,黄大鹏,靳鹏,李超..圈养西太平洋斑海豹口腔菌群多样性分析[J].中国动物检疫,2025,42(5):45-51,7.
