中国动物检疫2025，Vol.42Issue(5)：120-124,5.DOI:10.3969/j.issn.1005-944X.2025.05.019
非洲猪瘟病毒抗体阻断ELISA法测量不确定度评估
Measurement Uncertainty Evaluation on a Blocking ELISA for Antibodies Against ASFV
摘要
Abstract
In this study,blocking ELISA was used to detect antibodies against African swine fever virus(ASFV)for the purpose of evaluation on its reliability,of which measurement uncertainty was assessed by the"influence factor analysis"and the"control sample"method,respectively.Results showed that the expanded uncertainty values were determined as 0.053 0 and 0.044 2(k=2)by the two methods,indicating an overall low uncertainty of ELISA for ASFV antibodies on the basis of controlling the uncertainty that might be caused by the pipettes,incubators or microplate reader.No significant difference was observed between the uncertainty values evaluated by the two methods.Key factors influencing the results were obtained by the"influence factor analysis"method through analyzing relative contribution of each component to the results,thus supporting further improvement;while a large number of calculations might be reduced by the"control sample"method,which was more accessible.In conclusion,accurate results could be obtained by the application of uncertainty in ELISA assay for ASFV antibodies,contributing to the improvement of uncertainty evaluation system in veterinary testing.关键词
ELISA/非洲猪瘟病毒抗体/测量不确定度/"影响因素分析"法/"对照样品"法Key words
ELISA/ASFV antibody/measurement uncertainty/"influence factor analysis"method/"control sample"method分类
农业科技引用本文复制引用
柴娟,黄靖,郑方宇,谢荣辉,张传亮,张璐,董建斐,周丰超,王雅婷,孙仁杰,陈文静,黄晓兵,冯肖肖..非洲猪瘟病毒抗体阻断ELISA法测量不确定度评估[J].中国动物检疫,2025,42(5):120-124,5.基金项目
浙江省农业重大技术协同推广计划项目(2023ZDXT14-5) （2023ZDXT14-5）
浙江省"三农九方"科技协作项目(2023SNJF059) （2023SNJF059）
