非洲猪瘟病毒抗体阻断ELISA法测量不确定度评估OA

In this study,blocking ELISA was used to detect antibodies against African swine fever virus(ASFV)for the purpose of evaluation on its reliability,of which measurement uncertainty was assessed by the"influence factor analysis"and the"control sample"method,respectively.Results showed that the expanded uncertainty values were determined as 0.053 0 and 0.044 2(k=2)by the two methods,indicating an overall low uncertainty of ELISA for ASFV antibodies on the basis of controlling the uncertainty that might be caused by the pipettes,incubators or microplate reader.No significant difference was observed between the uncertainty values evaluated by the two methods.Key factors influencing the results were obtained by the"influence factor analysis"method through analyzing relative contribution of each component to the results,thus supporting further improvement;while a large number of calculations might be reduced by the"control sample"method,which was more accessible.In conclusion,accurate results could be obtained by the application of uncertainty in ELISA assay for ASFV antibodies,contributing to the improvement of uncertainty evaluation system in veterinary testing.