中国水产科学2025，Vol.32Issue(3)：299-311,13.DOI:10.12264/JFSC2024-0389
马口鱼卵巢周年发育与规模化人工繁殖
Annual ovary development and large-scale artificial reproduction of hooksnout carp,Opsariichthys bidens
摘要
Abstract
This study investigated the annual changes in ovary development in hooksnout carp and methods to improve artificial breeding and promote its rapid commercialization.The annual range in the gonadosomatic index(GSI)in female hooksnout carp in Hubei Province was 1.16％—11.65％.The spawning period was from mid-April to mid-July,and the average absolute fecundity of the female before spawning was 6165.4 eggs.In autumn,when the water temperature gradually decreased(September to October),the GSI of the ovaries gradually decreased,and the ovaries degenerated to phase Ⅲ and entered hibernation.In February of the following year,GSI of the carp was relatively low(1.16％—1.65％),and the proportion of phase Ⅲ oocytes in the ovary increased after a further decrease in water temperature to＜8.0 ℃ in February.In the scaled-up artificial breeding experiment,the combination of luteinizing releasing hormone(LRH-A2;5 μg/kg)and human chorionic gonadotropin hCG(1000 IU/kg)had the best induction effect of 87.0％.A 0.3％NaCl solution was the best fertilization medium,with an optimal sperm-to-egg ratio of 5.0×104-1.0×105∶1,which produced average fertilization and hatching rates of 66.6％and 80.1％,respectively.The induction time was 20.0-26.0 h before May 15,16.0-17.0 h after May 15,with accumulated temperature of 400.00-430.00 ℃·h.The hatching time was 69.0-90.0 h in water of 20.0-25.0 ℃,and 42.5-48.0 h in water＞26.0 ℃,with a hatching accumulated temperature of 1200.00-1300.00 ℃·h.This study also successfully conducted second-induced spawning of hooksnout carp broodstock in the same year and found that the spawning effect of the second breeding was not substantially different from that of the first.This study achieved scaled-up artificial breeding of hooksnout carp,laying an important foundation for the genetic improvement and commercialization of hooksnout carp.关键词
多不饱和脂肪酸/卵巢发育/人工催产/精子浓度/低渗溶液Key words
polyunsaturated fatty acids/ovarian development/large-scale induced spawning/sperm density/hypotonic solution分类
水产学引用本文复制引用
邓翔,邹聪,沈凡,乔浩峰,陈玉佩,胡晨昊,别汉晴,樊启学,沈志刚..马口鱼卵巢周年发育与规模化人工繁殖[J].中国水产科学,2025,32(3):299-311,13.基金项目
湖北省支持种业高质量发展资金项目(HBZY2023B009) （HBZY2023B009）
湖北省技术创新计划项目(2024BBB016). （2024BBB016）
