中国实验动物学报2025，Vol.33Issue(4)：467-478,12.DOI:10.3969/j.issn.1005-4847.2025.04.001
重症哮喘动物模型构建研究
Construction of a severe asthma animal model
摘要
Abstract
Objective To develop an animal model that replicates the clinical phenotype of severe asthma.Methods Ovalbumin(OVA)combined with IL-33 or varying doses of lipopolysaccharides(LPS)was used to explore the construction of a severe asthma mouse model.Established model animals were assessed for lung function,number of inflammatory cells,and lung tissue pathology were assessed.Expression of key genes associated with severe asthma identified from the GEO database were validated in the new model.Results Compared with OVA alone,OVA combined with IL-33 or 5 μg LPS significantly increased airway resistance and the number of inflammatory cells in bronchoalveolar lavage fluid,and aggravated the pathological damage to lung tissues.The expression patterns of key genes in the newly constructed severe asthma models were consistent with those observed in clinical patients with severe asthma.Conclusions The modeling method of combining OVA with IL-33 or LPS(5 μg)can be used to construct experimentalanimal models of severe asthma.关键词
脂多糖/卵清蛋白/重症哮喘/IL-33/GEO数据库Key words
lipopolysaccharides/ovalbumin/severe asthma/IL-33/GEO database分类
生物科学引用本文复制引用
杨迪,鹿振辉,蒋雨薇,李翠,马子风,王钰,陈林锦,陆天逊,崔洁..重症哮喘动物模型构建研究[J].中国实验动物学报,2025,33(4):467-478,12.基金项目
国家自然基金青年项目(82205004),上海市卫健委中医药科研项目(2022QN003),浦东新区中医药传承创新发展示范试点项目(YC-2023-0901).Funded by National Natural Science Foundation of China Youth Program(82205004),Shanghai Health Commission Traditional Chinese Medicine Research Project(2022QN003),Demonstration Pilot Project of Traditional Chinese Medicine Inheritance and Innovation Development in Pudong New Area(YC-2023-0901). （82205004）
