中国实验动物学报2025，Vol.33Issue(4)：479-488,10.DOI:10.3969/j.issn.1005-4847.2025.04.002
基于双人源化小鼠模型探索靶向间质MAOA在前列腺癌免疫微环境中的作用
Targeting of stromal MAOA expression in the immune microenvironment of prostate cancer using a dual-humanized mouse model
摘要
Abstract
Objective Construction of an immune-tumor dual humanized mouse model to explore the role of targeting monoamine oxidase A(MAOA)in the immune microenvironment of prostate cancer.Methods Bioinformatics analysis was used to examine the relationship between MAOAhigh cancer associated fibroblasts(CAFs)and T cells in prostate cancer.Multiplex immunofluorescence was employed to analyze the relationship between stromal MAOA expression and CD8+T cells.An immune-tumor dual humanized mouse model was constructed for in vivo verification of the infiltration of CD8+T cells in response to the targeting of stromal MAOA.Results MAOA expression in the stroma was inversely proportional to the infiltration of CD8+T cells.Inhibiting MAOA expression in the stroma enhanced the infiltration of CD8+T cells in vivo,which may reflect suppression of the accumulation of collagen in the tumor microenvironment.Conclusions Stromal MAOA plays an important role in the immunosuppressive microenvironment of prostate cancer,and its inhibition may promote the infiltration of immune cells.MAOA inhibitors have therapeutic potential in immune combination therapy for patients with prostate cancer.关键词
前列腺癌/MAOA/肿瘤相关成纤维细胞/肿瘤免疫微环境Key words
prostate cancer/MAOA/cancer associated fibroblasts/tumor immune microenvironment Conflicts of Interest:The authors declare no conflict of interest分类
生物学引用本文复制引用
赵志特,何兴林,师长宏,杨力军..基于双人源化小鼠模型探索靶向间质MAOA在前列腺癌免疫微环境中的作用[J].中国实验动物学报,2025,33(4):479-488,10.基金项目
国家自然科学基金(32270566),空军军医大学第一附属医院航空临床医学专项研究项目(HKLCZX03).Funded by the National Natural Science Foundation of China(32270566),the First Affiliated Hospital of Air Force Military Medical University Aviation Clinical Medicine Special Research Project(HKLCZX03). （32270566）
