中国实验动物学报2025，Vol.33Issue(4)：489-500,12.DOI:10.3969/j.issn.1005-4847.2025.04.003
干扰素-γ基因敲除小鼠饲养繁殖方法的优化
Optimization of feeding and breeding methods for interferon-γknockout mice
摘要
Abstract
Objective The purpose of the study is to breed homozygous interferon-γ knockout(IFN-γ-/-)mice and optimize the breeding strategies to achieve continuous and stable reproduction of IFN-γ-/-mice,which could be used as an ideal animal model for fundamental research.Methods Initially,heterozygous IFN-γ knockout(IFN-γ+/-)C57BL/6J mice were used as the parental generation for breeding.Subsequently,3 breeding strategies were employed using the offspring:(1)female heterozygotes mated with male heterozygotes;(2)male homozygotes mated with female heterozygotes;(3)female homozygotes mated with male homozygotes.The number and survival rate of IFN-γ-/-mice were compared across the three breeding strategies to determine the optimal breeding strategy.Under the optimal strategy,the effects of female mating age and diet type on the reproductive performance of IFN-γ-/-mice were further evaluated.Data from the first three litters of 60 IFN-γ-/-female mice,including litter size,number of weaning survivors,and weaning survival rate,were recorded and analyzed.In addition,the effects of dietary supplementation of pregnant mice and environmental optimization measures,such as the provision of shelters,were evaluated.Results Under conditions where the nutritional needs of pregnant mice were adequately met by supplementation with egg yolk and sunflower seeds,mating of female and male IFN-γ-/-mice result ed in a litter size of five to eight IFN-γ-/-mice,demonstrating higher efficiency compared to other breeding strategies.In addition,diet type and mating age significantly influenced female reproductive performance.When 7～9 weeks old female IFN-γ-/-mice were mated to male IFN-γ-/-mice and fed a high-protein breeding diet,litter size(6.9±1.7),weaning survival number(6.5％±2.0％)and weaning survival rate(93.2％±17.8％)were higher than those under other conditions.In addition,providing shelters to prevent fighting between breeding pairs further improved reproductive outcomes.Conclusions By adopting an optimized breeding strategy,combined with a high-protein diet,nutritional supplementation,and standardized mating age management,the breeding efficiency and stability of IFN-γ-/-mice can be significantly improved.This provides a reliable animal model for related research.关键词
干扰素-γ/基因敲除小鼠/饲养/繁殖Key words
interferon-γ/gene knockout mouse/feeding/reproduction分类
生物科学引用本文复制引用
柳慧敏,何茜,贾瑞莲,李娜,许瑞,冯耀宇,肖立华,郭亚琼..干扰素-γ基因敲除小鼠饲养繁殖方法的优化[J].中国实验动物学报,2025,33(4):489-500,12.基金项目
国家自然科学基金(32273032),广东省基础与应用基础研究基金(2024B1515020116).Funded by the National Natural Science Foundation of China(32273032),Guangdong Basic and Applied Basic Research Foundation(2024B1515020116). （32273032）
