中国实验动物学报2025，Vol.33Issue(4)：512-521,10.DOI:10.3969/j.issn.1005-4847.2025.04.005
抑肝扶脾汤调控PKA/PKC-CREB通路修复肠道屏障降低IBS敏感性的机制研究
Mechanistic study of Yigan Fupi decoction-mediated repair of the intestinal barrier and reduction of IBS sensitivity through regulation of the PKA/PKC-CREB pathway
摘要
Abstract
Objective To study the therapeutic effects of Yigan Fupi decoction(YGFP)on irritable bowel syndrome(IBS)and its mechanism of action in repairing the intestinal barrier and reducing IBS sensitivity through the PKA/PKC-CREB pathway.Methods Baby rats separated from their mother were randomly divided into a model control(M)and a YGFP group,while baby rats without maternal separation were used as a normal control(N)group.The YGFP group was given YGFP for 4 weeks.Abdominal withdrawal reflux was used to evaluate intestinal sensitivity.Liquid chromatography tandem mass spectrometry and ELISA were used to detect bile acid metabolite concentrations and serum levels of interleukin(IL)-6 and CXCL1,respectively.HE staining was used to observe pathological changes in the colon,and Western Blot and immunohistochemistry were used to analyze the relative protein expression levels of PKA,PKC,CREB,5HT2AR,5-HT7R,ZO-1,and Claudin 1.Results Compared with the normal control group,the M group showed a significantly decreased visceral pain threshold,significantly increased levels of total bile acid metabolites,IL-6,and CXCL1,significantly increased relative expression of PKA,PKC,CREB,5HT2AR,and 5-HT7R,and significantly decreased relative expression of ZO-1 and Claudin 1.Compared with the M group,the YGFP group showed a significantly increased visceral pain threshold,significantly reduced levels of total bile acid metabolites,IL-6,and CXCL1,significantly reduced relative expression of PKA,PKC,CREB,5HT2AR,and 5-HT7R,and increased relative expression of ZO-1 and Claudin 1.Conclusions YGFP effectively improved IBS through a mechanism that may involve repair of the intestinal barrier and reduced sensitivity through the PKA/PKC-CREB pathway.关键词
抑肝扶脾汤/肠道屏障/IBS敏感性/PKA/PKC/CREBKey words
Yigan Fupi decoction/intestinal barrier/IBS sensitivity/PKA/PKC/CREB分类
生物学引用本文复制引用
陈宇,施佳君,富丹婷,杨钦钦,傅睿,朱佳杰,朱明锦,刘忻颖,陈明显..抑肝扶脾汤调控PKA/PKC-CREB通路修复肠道屏障降低IBS敏感性的机制研究[J].中国实验动物学报,2025,33(4):512-521,10.基金项目
浙江省科技厅2024年院所专项(YSZX2401),浙江省自然科学基金(LY21H270003,ZCLMS25H2701).Funded by Zhejiang Provincial Department of Science and Technology Research Institute Support Program 2024(YSZX2401),Natural Science Foundation of Zhejiang Province(LY21H270003,ZCLMS25H2701). （YSZX2401）
