中国实验动物学报2025，Vol.33Issue(4)：522-529,8.DOI:10.3969/j.issn.1005-4847.2025.04.006
黄芩通过JAK2/STAT3信号通路联合5-Fu发挥抗C57BL/6J小鼠肺癌的作用
Scutellaria baicalensis exerts its anti C57BL/6J mouse lung cancer effect through the JAK2/STAT3 signaling pathway combined with 5-Fu
摘要
Abstract
Objective To investigate the inhibitory effect of Scutellaria baicalensis combined with 5-Fu on Lewis tumor bearing mouse lung cancer based on the JAK2/STAT3 signaling pathway.Methods To detect the changes in body mass,food intake and tumor volume of Lewis tumor-bearing mice after intervention of Scutellaria baicalensis(20 mg/kg)combined with 5-Fu.Pathological changes in tumor tissue were observed by HE staining,expression levels of proliferation related protein Ki67 were observed by immunohistochemical staining,changes in cell apoptosis levels in tumor tissue were observed by TUNEL staining,and changes in expression levels of JAK2,p-JAK2,STAT3,and p-STAT3 proteins in tumor tissue were observed by immunohistochemical staining.Results Compared with the model group,the tumor volume of mice after combined intervention significantly decreased(P＜0.01)and body mass increased(P＜0.05),but there was no significant change food intake.The expression of proliferation related protein Ki67 in tumor tissue was significantly reduced,and the number of apoptotic cells labeled with TUNEL was significantly increased;The expression of p-JAK2 and p-STAT3 proteins is elevated.Conclusions Scutellaria baicalensis decoction can inhibit the JAK2/STAT3 signaling pathway and increase the inhibitory effect of 5-Fu on mouse lung cancer.关键词
肺癌荷瘤小鼠/黄芩/JAK2/STAT3信号通路/5-FuKey words
lung cancer bearing tumor mice/Scutellaria baicalensis/JAK2/STAT3 signaling pathway/5-Fu Conflicts of Interest:The authors declare no conflict of interest分类
生物学引用本文复制引用
龙亚丽,刘雅莉,杨明轩,田启会,张勇..黄芩通过JAK2/STAT3信号通路联合5-Fu发挥抗C57BL/6J小鼠肺癌的作用[J].中国实验动物学报,2025,33(4):522-529,8.基金项目
武威市自然科学基金(WW23A03RPZ007),2024年甘肃省高校教师创新基金项目(2024A303),武威市科技计划B类项目(WW23B02NY119).Funded by National Natural Science Foundation of Wuwei(WW23A03RPZ007),2024 Gansu Province University Teacher Innovation Fund Project(2024A303),Wuwei City Science and Technology Plan Class B Project(WW23B02NY119). （WW23A03RPZ007）
