中国实验动物学报2025，Vol.33Issue(4)：530-539,10.DOI:10.3969/j.issn.1005-4847.2025.04.007
唾液乳杆菌Li01对幽门螺杆菌合并苯并芘致长爪沙鼠DNA损伤的改善作用研究
Therapeutic effects of Ligilactobacillus salivarius Li01 on DNA damage induced by the combination of Helicobacter pylori and Benzo(a)pyrene in Mongolian gerbils
摘要
Abstract
Objective To investigate the protective effects of Ligilactobacillus salivarius Li01(L.salivarius Li01)against DNA damage induced by Helicobacter pylori(Hp),Benzo(a)pyrene(BaP),and Hp+BaP,and to evaluate the probiotic properties of L.salivarius Li01.Methods After 1 week of adaptive feeding,specific pathogen-free male Mongolian gerbils were randomly assigned to groups and subjected to intragastric administration of Hp,BaP,and Hp+BaP for model induction.At week 32 post model establishment,therapeutic L.salivarius Li01 was administered intragastrically.At week 36,peripheral blood samples were collected from each group for the comet assay,while liver tissues were collected and tested for Cyp1a1 gene expression levels.Results Compared with those in the control group,the comet tail length,％tail DNA,and hepatic Cyp1a1 expression levels were significantly increased in the Hp,BaP,and Hp+BaP groups(P＜0.0001).Among these,the comet tail length,olive tail moment,％tail DNA,and hepatic Cyp1a1 expression levels were significantly higher in the Hp+BaP group than in the Hp and BaP groups(P＜0.05).Following intervention with L.salivarius Li01,the comet tail length,olive tail moment,％tail DNA,and hepatic Cyp1a1 expression levels were significantly reduced in each group(P＜0.001).Conclusions Hp infection,BaP exposure,and the Hp+BaP combination induced DNA damage in the peripheral lymphocytes of Mongolian gerbils,with the Hp+BaP combination showing synergistic damage.L.salivarius Li01 had a protective effect against DNA damage caused by Hp,BaP,and Hp+BaP.关键词
唾液乳杆菌/幽门螺杆菌/苯并芘/长爪沙鼠模型/DNA损伤Key words
Ligilactobacillus salivarius/Helicobacter pylori/Benzo(a)pyrene/Mongolian gerbil model/DNA damage分类
生物学引用本文复制引用
黄逸伦,徐芝炫,郭红刚,张扬帆,刘慧敏,谢文迪,陈永平,褚晓峰..唾液乳杆菌Li01对幽门螺杆菌合并苯并芘致长爪沙鼠DNA损伤的改善作用研究[J].中国实验动物学报,2025,33(4):530-539,10.基金项目
2024年温州医科大学本专科学生科研立项资助课题(wyx2024101143),浙江省基础公益项目(LGD22C040024).Funded by 2024 Wenzhou Medical University Undergraduate and Junior College Student Research Funding Projects(wyx2024101143),the Basic Public Welfare Research Program of Zhejiang Province(LGD22C040024). （wyx2024101143）
