中国比较医学杂志2025，Vol.35Issue(4)：11-20,10.DOI:10.3969/j.issn.1671-7856.2025.04.002
毛兰素通过调节HMGB1/RAGE-RhoA/ROCK1信号通路缓解特应性皮炎
Erianin alleviates atopic dermatitis by regulating the HMGB1/RAGE-RhoA/ROCK1 signaling pathway
摘要
Abstract
Objective To explore the role of Erianin in atopic dermatitis(AD)and its regulatory mechanism involving the high-mobility group box 1(HMGB1)/receptor for advanced glycation end products(RAGE)-Ras homolog gene family member A(RhoA)/Rho-associated protein kinase 1(ROCK1)signaling pathway.Methods An AD model was induced in BALB/c mice using 1-chloro-2,4-dinitrobenzene(DNCB).Skin thickness and spleen and lymph node weight were measured and pathological changes in the back skin and ears were detected using methylamine blue and hematoxylin and eosin staining.Inflammatory factors were detected by enzyme-linked immunosorbent assay.An in vitro model of AD was established in HaCaT cells stimulated by tumor necrosis factor(TNF)-α.Cellular reactive oxygen species(ROS)were detected by flow cytometry and mitochondrial ROS(mtROS)were detected by immunofluorescence assay.Cell apoptosis was detected by terminal deoxynucleotidyl transferase dUTP nick-end labeling.HMGB1,RAGE,RhoA,and ROCK1 proteins were detected by Western blot.Results Erianin inhibited the increase in skin thickness,reduced the spleen and lymph node weights,improved the infiltration of inflammatory cells and the degranulation of mast cells,and reduced the levels of inflammatory factors(P＜0.05).Erianin also reduced the production of cellular ROS and mtROS induced by TNF-α in vitro(P＜0.01),and decreased the protein expression of HMGB1,RAGE,RhoA,and ROCK1(P＜0.01).Treatment of HMGB1-stimulated HaCaT cells with a RAGE-specific blocker(TFA)had no effect on HMGB1 expression,while expression levels of RAGE,RhoA,and ROCK1 were decreased(P＜0.01).Cells treated with the Rho kinase inhibitor Y-27632+r-HMGB1 group showed similar result to the TFA+r-HMGB1 group,except for RAGE.Conclusions Erianin relieves AD by regulating the HMGB1/RAGE-RhoA/ROCK signaling pathway.关键词
毛兰素/特应性皮炎/HMGB1/RAGE/RHOA/ROCK1Key words
Erianin/atopic dermatitis/HMGB1/RAGE/RhoA/ROCK1引用本文复制引用
徐可欣,王丹丹,金泓宇,杜月,李莉,宋艺兰,延光海,李良昌..毛兰素通过调节HMGB1/RAGE-RhoA/ROCK1信号通路缓解特应性皮炎[J].中国比较医学杂志,2025,35(4):11-20,10.基金项目
国家自然科学基金(82060008) （82060008）
吉林省科技厅项目(YDZJ202201ZYTS140). （YDZJ202201ZYTS140）
