中国实用口腔科杂志2025，Vol.18Issue(3)：300-306,7.DOI:10.19538/j.kq.2025.03.008
不同防龋试剂对早期邻面龋再矿化及抗菌作用效果的对比研究
Comparative study on the effect of different anti-caries reagents on remineralization and antimicrobial proper-ties of early adjacent caries
摘要
Abstract
Objective To study the effects of different anti-caries reagents[selenium-doped bioactive glass(SBG),bio-active glass(BG)and sodium fluoride(NaF)].n the remineralization and antibacterial properties of early adjacent caries,so as to provide a theoretical basis for rational drug use in caries prevention and treatment.Methods Totally 48 premo-lars removed for orthodontic treatment in Shenyang Stomatological Hospital from June to August 2023 were selected to es-tablish an early adjacent caries model(96 samples were obtained).All sample models were randomly divided into SBG group,BG group,NaF group and blank control group(24 in each group).The first three groups were remineralized with 5％SBG sol,5％BG sol and Dulefluoride respective-ly,while the blank control group was not remineralized.Microhardness tester,white light interferometer,scanning electron microscope(SEM)and energy dispersive spec-trometer(EDS)were used to analyze the microhardness of demineralized and remineralized enamel surface,as well as the morphology and structure of remineralized enamel surface and the mass ratio of calcium and phosphorus after remineral-ization.In addition,three kinds of anti-caries reagents(SBG group,BG group and NaF group)were used to contact and co-culture with Streptococcus mutans(S.mutans),and their antibacterial effects on S.mutans were observed by bacterio-static ring method,direct contact method and confocal laser scanning microscope(CLSM).Results There was no signif-icant difference in the microhardness values of each group after demineralization(F=0.716,P=0.457).After remineral-ization,the enamel surface microhardness of SBG group was significantly higher than that of BG group,NaF group and blank control group,and the calcium-phosphorus mass ratio(2.21±0.07)was significantly better than that of BG group(2.12±0.08),NaF group(1.85±0.06)and blank control group(1.56±0.03),the differences being statistically signifi-cant(all P＜0.05).White light interferometer and SEM showed that the enamel surface sediment of SBG group was dens-er after remineralization,and the depth of demineralization sag was uniform,indicating that its remineralization effect was significantly better than that of the other three groups.In addition,the diameter of the zone of inhibition[(18.70±1.39)mm]against S.mutans in the SBG group was significantly larger than that in the BG group[(12.02±1.64)mm]and NaF group[(7.53±0.87)mm],and the colony count[(13.33±2.49)CFU]was significantly lower than that in the BG group[(55.67±1.33)CFU]and NaF group[(252.33±5.00)CFU].CLSM showed that the percentage of viable plaque in the SBG group[(57.80±3.10)％]was significantly lower than that in the BG group[(63.70±1.74)％]and NaF group[(77.40±1.98)％],and the differences were statistically significant(all P＜0.05).Conclusion As a new bioac-tive material,SBG may be superior to BG and fluoride in promoting the remineralization and antibacterial performance of early adjacent caries.关键词
邻面龋/生物活性玻璃/硒/氟化钠/再矿化/抗菌性能Key words
adjacent caries/bioactive glass/selenium/sodium fluoride/remineralization/antimicrobial properties分类
口腔医学引用本文复制引用
吴嘉锜,刘文彬,魏丽冉,刘美霞,徐延,张桂荣..不同防龋试剂对早期邻面龋再矿化及抗菌作用效果的对比研究[J].中国实用口腔科杂志,2025,18(3):300-306,7.基金项目
沈阳市科技计划项目(23-408-3-02) （23-408-3-02）
