针灸临床杂志2025，Vol.41Issue(5)：68-73,6.DOI:10.19917/j.cnki.1005-0779.025095
电针干预对膝骨关节炎兔软骨细胞铁死亡相关蛋白表达的影响
Effects of EA Intervention on Ferroptosis-Related Protein Expressions in Chondrocytes of KOA Rabbits
摘要
Abstract
Objective:To observe the effect of electro-acupuncture(EA)intervention on the ferroptosis-related protein expressions of SLC7A11 and GPX 4,as well as on the level of GSH in rats with knee osteoarthritis(KOA).Methods:35 rabbits were randomly divided into the normal group,the model group,the model inhibitor group,the EA group,and the EA inhibitor group,with 7 rats in each group.The KOA model was eatablished using the modified Videman immobilization method with the left hind limb in an extended position.The normal group and the model group received no intervention,whereas the EA group and the EA hihibitor group were treated for 3 weeks.Transmission electron microscopy was used to observe the morphological changes in chondrocytes and mitochondria.Western blot method was performed to detect the protein expressions of SLC7A11 and GPX 4 in the knee joint cartilage.Biochemical method was used to measure the GSH content in the knee joint cartilage.Results:Compared with the normal group,the model group exhibited chondrocyte membrane rupture,organelle swelling,increased mitochondrial membrane density,reduced or absent cristae,and outer membrane ruptured;the protein expressions of SLC7A11 and GPX 4 were significantly decreased(P＜0.01),and the level of GSH was also significantly reduced in the model group compared to those in the normal group(P＜0.01).Compared with the model group,the EA group exhibited normal chondrocyte and mitochondrial morphology,with increased levels of SLC7A11,GPX 4 and GSH(P＜0.01).Compared with their respective intervention groups,the model inhibitor group and the EA hihibitor group showed decreased levels of SLC7A11,GPX 4 and GSH(P＜0.01,P＜0.05).Conclusion:EA intervention can improve the pathological state of chondrocyte ferroptosis,which may be related to up-regualting the ferroptosis-related proteins expressions of SLC7A11,GPX 4 and GSH.关键词
膝骨关节炎/电针/铁死亡/SLC7A11/GPX 4/GSHKey words
Knee osteoarthritis/Electro-acupuncture/Ferroptosis/SLC7A11/GPX 4/GSH分类
中医学引用本文复制引用
马芸瑄,郭长青,郭妍,邢龙飞..电针干预对膝骨关节炎兔软骨细胞铁死亡相关蛋白表达的影响[J].针灸临床杂志,2025,41(5):68-73,6.基金项目
国家自然科学基金面上项目,编号:82074523. （）
