肿瘤药学2025，Vol.15Issue(2)：187-196,10.DOI:10.3969/j.issn.2095-1264.2025.02.06
氯胍联合奥希替尼抑制膀胱癌细胞的增殖和迁移
The combination of proguanil and osimertinib inhibits the proliferation and migration of bladder cancer cells
摘要
Abstract
Objective To explore the impact of proguanil and osimertinib,both as individual treatments and in combina-tion,on the migratory behavior of bladder cancer cells,as well as to elucidate the potential mechanisms underlying these ef-fects.Methods MTT assays were conducted to assess the impact of proguanil and osimertinib as single agents on the prolif-eration of bladder cancer cells.Cell scratch assays and Transwell assays were employed to evaluate the effects of proguanil and osimertinib,both individually and in combination,on the migration of bladder cancer cells.Western blotting analysis was used to examine the effects of proguanil and osimertinib in combination on the expressions of epidermal growth factor receptor(EGFR)and epithelial-mesenchymal transition(EMT)markers,specifically E-cadherin and N-cadherin.Results Monotherapy with either proguanil or osimertinib significantly suppressed the proliferation and migration of blad-der cancer cells.The combination treatment of proguanil and osimertinib demonstrated a marked synergistic inhibiting effect,resulting in enhanced inhibition of both cell proliferation and migration compared to single-agent treatment.The combination treatment significantly inhibited EGFR phosphorylation,downregulated N-cadherin expression,and upregulat-ed E-cadherin expression.Conclusion The combination of proguanil and osimertinib effectively suppresses the prolifera-tion and migration of bladder cancer cells via inhibiting EGFR phosphorylation and blocking the EMT process.关键词
膀胱癌/奥希替尼/氯胍/EGFR/EMTKey words
Bladder cancer/Osimertinib/Proguanil/EGFR/EMT分类
医药卫生引用本文复制引用
王伟帆,周晓辰,谢宜君,钟奇,杨小平..氯胍联合奥希替尼抑制膀胱癌细胞的增殖和迁移[J].肿瘤药学,2025,15(2):187-196,10.基金项目
湖南师范大学生殖健康与转化医学研究团队资助项目(2023JC101). （2023JC101）
