植物保护学报2025，Vol.52Issue(2)：294-306,13.DOI:10.13802/j.cnki.zwbhxb.2025.2024079
山东省七个市小麦条锈菌群体的毒性结构和遗传多样性分析
Analysis of virulence structure and genetic diversity of wheat stripe rust fungus in seven cities of Shandong Province
摘要
Abstract
This study provides references for the control of wheat stripe rust and the rational layout of wheat resistance genes in the Huang-Huai-Hai wheat-growing region.A total of 152 isolates were col-lected from seven cities in Shandong Province,including Tai'an,Jinan and Weifang.The virulence phe-notyping was conducted using both Chinese differentials and Yr single-gene differentials.Meanwhile,17 pairs of kompetitive allele specific PCR-single nucleotide polymorphism(KASP-SNP)primers were used for genotype analysis to identify their genetic structure and diversity.The results showed that 152 wheat stripe rust pathotypes were obtained from the 152 strains of Puccinia striiformis f.sp.tritici(Pst)after isolation and pathotype expansion.According to the identification by Chinese differentials,among the 152 Pst strains,23 were known races and 27 were new races,among which Guinong 22 group was the dominant group of Pst in Shandong Province.The identification by Yr single-gene differentials revealed that the 152 Pst had 131 pathotypes,with race1(2.6%)being the predominant pathogenic type.The virulence frequency of Pst population to AvSYr32NIL(carrying resistance gene Yr32)was low,and none of them infected AvSYr5NIL(carrying resistance gene Yr5)and AvSYr15NIL(carrying resistance gene Yr15)wheat varieties.This indicates that wheat varieties carrying these resistance genes can still be used in the layout of wheat stripe rust resistance genes and disease-resistant breeding in Shandong Province.Cluster analysis based on KASP-SNP molecular marker data showed that there were similar genetic structures between Tai'an and Jinan populations,Zaozhuang and Linyi populations,and Wei-fang and Yantai populations.However,these six populations had a larger genetic structure difference with Heze population,which was similar to the clustering results based on the two sets of differentials.Genetic structure analysis,discriminant analysis of principal component(DAPC)and principal coordi-nate analysis(PCoA)all showed that there was gene flow within the Pst populations of the seven cities in Shandong Province,including Tai'an.96%of the genetic variation of the Pst populations in the seven cities occurred within the populations,and only 4%of the variation occurred between popula-tions,indicating that the genetic variation among individuals within the population was the main reason for the genetic variation of the Pst in the seven cities.There was a low correlation between the virulence and genotype of the Pst populations in the seven cities of Shandong Province,and no correlation between genetic distance and spatial geographical distance.关键词
小麦条锈菌/山东省/毒性鉴定/群体遗传Key words
Puccinia striiformis f.sp.tritici/Shandong Province/virulence identification/population genetics引用本文复制引用
高新培,靳永瑾,刘博凡,张珂,康振生,詹刚明..山东省七个市小麦条锈菌群体的毒性结构和遗传多样性分析[J].植物保护学报,2025,52(2):294-306,13.基金项目
国家重点研发计划(2021YFD1401000),国家自然科学基金(32172380) （2021YFD1401000）
