植物保护学报2025，Vol.52Issue(2)：366-378,13.DOI:10.13802/j.cnki.zwbhxb.2025.2024123
小麦茎基腐病生防菌P61的分离鉴定及发酵条件筛选
Isolation and identification of biocontrol bacterium P61 against wheat stem rot and screening of fermentation conditions
摘要
Abstract
To screen antagonistic bacterial strains against wheat crown rot,rhizosphere soil samples from Hebei Province wheat fields were collected for bacterial isolation.Fusarium pseudograminearum was selected as the target pathogen for preliminary biocontrol strain selection using dual-culture plate assays.Comprehensive characterization of the selected strain P61 was conducted through morphologi-cal observation,physiological-biochemical profiling,and molecular identification.Subsequent fermenta-tion optimization employed single-factor experiments,Plackett-Burman design,and response surface methodology.The results demonstrated that 11 bacterial isolates were obtained,with strain P61 exhibit-ing the strongest antifungal activity(79.88%inhibition rate)and identified as Bacillus amyloliquefa-ciens.The fermentation filtrate of P61 showed 78.86%suppression rate on F.pseudograminearum conidial germination,and demonstrated enzymatic activities including esterase,cellulase,amylase,pro-tease,and organic acid production,while displaying broad-spectrum antagonism against multiple Fusarium species.Through fermentation optimization,the optimal medium composition was deter-mined as mannitol 7 g/L,peptone 7 g/L,and MgSO4 4.31 g/L.The ideal fermentation parameters com-prised of 3%inoculation volume,40%liquid loading volume,186 r/min agitation,30.9℃incubation temperature,44.83 h cultivation duration,and pH 7.0.Under fermentation conditions,P61 achieved a biomass of 6.533×109 CFU/mL with 9.47%enhancement in antifungal efficacy against F.pseudogra-minearum.These findings suggest that B.amyloliquefaciens P61 is a promising biocontrol agent for wheat crown rot management.关键词
小麦茎基腐病/假禾谷镰孢菌/解淀粉芽胞杆菌/响应曲面分析法/发酵配方/发酵条件Key words
wheat stem basal rot/Fusarium pseudograminearum/Bacillus amyloliquefaciens/response surface methodology/fermentation formulation/fermentation condition引用本文复制引用
孙昊,董素芬,李雪,张佳琪,李满,李星,康占海..小麦茎基腐病生防菌P61的分离鉴定及发酵条件筛选[J].植物保护学报,2025,52(2):366-378,13.基金项目
河北省教育厅2023年度引进留学人员资助项目(C20230107) （C20230107）
