植物保护学报2025，Vol.52Issue(2)：400-407,8.DOI:10.13802/j.cnki.zwbhxb.2025.2024124
梨小食心虫谷氧还蛋白3调控雄成虫生殖的功能分析
Functional analysis of glutaredoxin 3 in regulating male reproduction of oriental fruit moth Grapholita molesta
摘要
Abstract
To investigate the potential role of glutaredoxin 3(Grx3)in the male reproduction of oriental fruit moth Grapholita molesta,a major global fruit-boring pest,the expression of the GmGrx3 gene was analyzed using real-time quantitative PCR in male accessory glands across different adult ages(newly emerged,1,2,3,and 4 days old)and different mating frequencies(1-4 matings).RNA interference(RNAi)and physiological/biochemical assays were further employed to assess the impact of GmGrx3 knockdown on male reproductive performance.The GmGrx3 gene was successfully cloned and charac-terized,encoding a protein with typical glutaredoxin structural domain,catalytic motifs,and active sites.Its expression peaked in 2-day-old male accessory glands,significantly higher than at other ages,but showed no significant variation across mating frequencies.Following RNAi-mediated knockdown of GmGrx3,males exhibited a significant reduction in female oviposition and egg hatching rate after mating.Additionally,levels of hydrogen peroxide and malondialdehyde in spermatophores were signifi-cantly elevated,while eupyrene sperm viability was markedly reduced,indicating substantial oxidative damage.These data demonstrate that GmGrx3 plays a crucial role in protecting eupyrene sperm from oxidative stress within the spermatophore,thereby contributing to male fertility in G.molesta.关键词
梨小食心虫/谷氧还蛋白3/雄成虫生殖/氧化应激/有核精子活率Key words
Grapholita molesta/glutaredoxin 3/male reproduction/oxidative stress/eupyrene sperm viability引用本文复制引用
程杰,魏巍,郭泰,张晓伟,相会明,马瑞燕..梨小食心虫谷氧还蛋白3调控雄成虫生殖的功能分析[J].植物保护学报,2025,52(2):400-407,8.基金项目
国家重点研发计划(2023YFD1401400),山西省基础研究计划(202403021212069),山西省重点研发计划(202302140601011) （2023YFD1401400）
