植物保护学报2025，Vol.52Issue(2)：408-417,10.DOI:10.13802/j.cnki.zwbhxb.2025.2024125
桃红颈天牛雌雄成虫转录组差异分析
Transcriptomic analysis of differential gene expression between female and male adults of red-necked longicorn Aromia bungii
摘要
Abstract
To identify differentially expressed genes(DEGs)and their associated biological functions between male and female adults of red-necked longicorn Aromia bungii,high-throughput transcriptome sequencing was conducted.The sequencing data were subjected to functional annotation and enrich-ment analyses based on Gene Ontology(GO)and Kyoto Encyclopedia of Genes and Genomes(KEGG)databases.A total of 31 077 unigenes were assembled,of which 21 613 were successfully annotated across seven major databases(NR,KOG,KEGG,GO,SwissProt,NT,and PFAM),with the NR database yielding the most annotations(18 201 unigenes).Comparative analysis revealed 6 944 DEGs between male and female adults,including 3 309 genes significantly up-regulated and 3 635 genes were significantly down-regulated in females relative to males.Functional enrichment analyses of these DEGs led to the identification of eight reproduction-related genes with potential as novel targets for pest control,including genes encoding vitellogenin,vitellogenin receptor,steroidogenic acute regulatory pro-tein,cyclin-b3,seminal fluid protein,spermatogenesis-associated protein 6,hyaluronidase and cAMP-dependent protein kinase.关键词
桃红颈天牛/转录组/高通量测序/差异表达基因/害虫防治/功能注释Key words
Aromia bungii/transcriptome/high-throughput sequencing/differentially expressed gene/pest management/functional annotation引用本文复制引用
戚煜,刘欣玉,白妍妍,赵盈盈,谢寿安,王永平..桃红颈天牛雌雄成虫转录组差异分析[J].植物保护学报,2025,52(2):408-417,10.基金项目
国家公益性行业(林业)项目(201504324),陕西省第二批特支计划领军人才项目(陕组通字2020-44号),2024年陕西林业科技创新专项(SXLK2024-0213) （林业）
