中国中西医结合杂志2025，Vol.45Issue(5)：609-615,7.DOI:10.7661/j.cjim.20250114.065
基于转录组学探讨清眩润目饮干预苯扎氯铵诱导干眼小鼠作用机制
Mechanism of Qingxuan Runmu Yin on Benzalkonium Chloride Induced Dry Eye Mice Based on Transcriptomics
摘要
Abstract
Objective To investigate the therapeutic effects of Qingxuan Runmu Yin(QXRMY)on benzalkonium chloride(BAC)-induced dry eye disease(DED)in mice and explores its molecular mechanisms using transcriptome sequencing(RNA-seq).Methods Sixty C57BL/6J mice were randomly divided into blank group,model group,and QXRMY group,with 20 mice in each group.The DED model was established in the model and QXRMY groups using BAC eye drops.Mice in the QXRMY group received QXRMY(17.61 mg·kg-1·d-1),while the blank and model groups were given an equivalent amount of physiological saline for two weeks.Tear secretion(Schirmer's test,SIT)and corneal fluorescein sodium staining scores(CFS)were measured.Histopathological changes in tissues were observed using HE staining,while PAS staining was used to assess goblet cell alterations.RNA-seq was performed to detect gene expression profiles in the cornea,identify differentially expressed genes(DEGs),and conduct gene ontology(GO),Kyoto encyclopedia of genes and genomes(KEGG)pathway,and protein-protein interaction(PPI)network analyses.Core gene expression was validated using RT-qPCR.Results QXRMY significantly improved corneal and conjunctival damage in DED mice,increased tear secretion(P＜0.01),and reduced CFS scores(P＜0.01).RNA-seq analysis revealed 361 DEGs reversed by QXRMY treatment,with significant enrichment in biological processes such as inflammatory and immune responses.KEGG pathway analysis highlighted key pathways,including IL-17 and chemokine signaling.PPI network analysis identified core genes such as interleukin-1β(IL-1β),matrix metalloprotein 9(Mmp9),tissue inhibitor of metal protease 1(Timp1),and chemokine C-C motif ligand 3(Ccl3).RT-qPCR validation confirmed that compared with the blank group,the mRNA expression levels of IL-1β,Ccl3,Mmp9,Timp1,and Mmp9/Timp1 in the model group increased(P＜0.01).Compared with the model group,the mRNA expression levels of IL-1β,Mmp9,Timp1,Ccl3,and Mmp9/Timp1 in the QXRMY group decreased(P＜0.05,P＜0.01).Conclusions QXRMY effectively alleviates ocular surface symptoms in BAC-induced DED mice.Its molecular mechanism may be related to pathways and genes related to the regulation of inflammatory factors,chemokines and metalloproteinase synthesis.关键词
清眩润目饮/干眼/蒸发过强型干眼/转录组测序/苯扎氯铵/分子机制/中药复方/中西医结合Key words
Qingxuan Runmu Yin/dry eye/evaporative dry eye/RNA-seq/benzalkonium chloride/molecular mechanism/Chinese herbal compound/integrative medicine引用本文复制引用
赵珊珊,张致睿,刘颖,姚靖..基于转录组学探讨清眩润目饮干预苯扎氯铵诱导干眼小鼠作用机制[J].中国中西医结合杂志,2025,45(5):609-615,7.基金项目
国家自然科学基金资助项目(No.81973908) （No.81973908）
黑龙江省中医药经典普及化专项课题项目(No.ZYW2022-051) （No.ZYW2022-051）
