遵义医科大学学报2025，Vol.48Issue(5)：455-460,6.
肉桂醛抗结核分枝杆菌作用及胞内杀菌机制的研究
Research on the anti-mycobacterium tuberculosis effect of cinnamaldehyde and its mechanism of intracellular bactericidal activity
摘要
Abstract
Objective To investigate the antibacterial activity of cinnamaldehyde against Mycobacterium tubercu-losis(MTB),and to further understand the intracellular bactericidal effect of cinnamaldehyde and its mecha-nism.Methods Determination of the minimum inhibitory concentration(MIC)and the minimum bactericidal concentration(MBC)of cinnamaldehyde against Mycobacterium tuberculosis standard strain H37Rv by applying microdilution resazurin titration;The intracellular bactericidal effect of cinnamaldehyde was assessed by counting intracellular viable colonies;Lactic dehydrogenase(LDH)assay was used to detect the cytotoxic effect of cinna-maldehyde;Flow cytometry was used to detect the apoptosis of macrophages after H37Rv infection treated with cinnamaldehyde,and Western blot(WB)was used to detect the changes of apoptosis and autophagy of macro-phages after cinnamaldehyde treatment.Results Cinnamaldehyde had a strong extracellular antibacterial and bac-tericidal activity against H37Rv with MIC of 59 μmol/L and MBC of 59-118 μmol/L;Cinnamaldehyde has an intracellular bactericidal activity against H37Rv infected macrophages,and has no obvious cytotoxicity at the minimum inhibitory concentration;Flow cytometry and WB showed that cinnamaldehyde promoted the apoptosis of macrophages after H37Rv infection.Conclusion Cinnamaldehyde has bactericidal activity against Mycobacteri-um tuberculosis,and may play an intracellular role by inducing macrophage apoptosis.关键词
肉桂醛/结核分枝杆菌/巨噬细胞/胞内杀菌/凋亡Key words
cinnamaldehyde/mycobacterium tuberculosis/macrophages/intracellular bactericidal activity/apoptosis分类
药学引用本文复制引用
吴霞,彭章丽,刘梅,张万敏,王小丹,娄思玉,陈玲..肉桂醛抗结核分枝杆菌作用及胞内杀菌机制的研究[J].遵义医科大学学报,2025,48(5):455-460,6.基金项目
遵义医科大学博士启动资金资助项目[NO:院字(2024)13号] （2024）
遵义市科技计划资助项目[NO:遵市科合HZ字(2023)96号,遵市科合HZ字(2022)254号]. （2023）
