中医肿瘤学杂志2025，Vol.7Issue(3)：30-42,13.DOI:10.19811/j.cnki.ISSN2096-6628.2025.03.005
黄腻苔结直肠癌患者肠道微环境代谢特征研究
Study on the Metabolic Characteristics of Intestinal Microenvironment in Colorectal Cancer Patients with Yellow Greasy Tongue Coating
摘要
Abstract
Objective To analyze the metabolic characteristics of tumor microenvironment in colorectal cancer(CRC)patients with yellow greasy tongue coating(YGTC).Methods Twelve CRC patients with YGTC and nine patients with white greasy tongue coating(WGTC),as diagnosed by tongue diagnosis instruments,were randomly selected.Cancerous and adjacent non-cancerous tissues from these patients were analyzed using metabolomics and transcriptomics.Additionally,six CRC patients with YGTC and six CRC patients with WGTC were collected,and the previously sequenced results were validated through experiments of Oil Red O staining,Western blot,PCR,MDA detection,and ROS detection.Results Metabolomics and transcriptomics analyses revealed lipid metabolism disorders in CRC patients with greasy tongue coating.The key distinguishing feature of YGTC different from WGTC was the downregulation of fatty acid degradation pathway.Up-regulation of acylcarnitine,a key intermediate in fatty acid metabolism,was observed in CRC patients with YGTC,reflecting disruption of fatty acid degradation.Conclusion The changes in yellow and white greasy tongue coating of CRC patients can reflect the metabolic characteristics of intestinal microenvironment关键词
结直肠癌/舌苔/代谢组学/转录组学Key words
colorectal cancer/tongue coating/metabolomics/transcriptomics分类
中医学引用本文复制引用
刘雪丽,吕琨,蒋寒冰,朱跃坤,徐巍..黄腻苔结直肠癌患者肠道微环境代谢特征研究[J].中医肿瘤学杂志,2025,7(3):30-42,13.基金项目
中西医协同"旗舰"科室建设项目(编号:国中医药综结合函[2024]221号) （编号:国中医药综结合函[2024]221号）
中医优势专科建设项目(编号:黑中医药综涵[2022]36号). （编号:黑中医药综涵[2022]36号）
