摘要
Abstract
OBJECTIVE:To use network pharmacology analysis together with molecular docking and in vitro cell experiments for evaluating therapeutic potential of ginseng cyclopentadienol(PND)on Alzheimers disease,and for understanding its protective effect on neurons.METHODS:Targets of PND in the treatment of Alzheimers disease were screened based on PharmMapper,GeneCards,STRING and other databases,and a protein interaction network was constructed.Characteristic pathways were identified by GO and KEGG pathway enrichment analysis and then verified by molecular docking technology.An L-glutamate-induced cell injury model was established using human neuroblastoma SH-SY5Y cells.After treatment of these cells with different concentrations of PND(10,20,40 μmol/L),cell activity was detected by CCK-8 method,and apoptosis was detected by flow cytometry.RESULTS:A PND-core target-characteristic pathway-Alzheimers disease network was constructed,and the important targets regulated by PND were found to be ALB,AKT1,EGFR,SRC,CASP3,NFKB1,PTGS2,PPARG,etc.Multiple pathways were identified to involve EGFR,C-type lectin receptor signaling,RAS signaling,PI3K-Akt signaling and Alzheimers disease signaling.The molecular docking results showed that PND had a good regulatory effect on targets such as CYP3A4,APP,and EGFR.Results from the in vitro cell experiments showed that the survival rate of SH-SY5Y cells in the L-glutamate-induced nerve injury model group was reduced,and the cell survival rate in the 20 μmol/L PND treatment group was higher than that in the model group(P<0.01);the total apoptosis rate of nerve cells in the model group was 45.23%,and the apoptosis rates of cells in the 10,20 and 40 μmol/L PND treatment groups were 41.34%,36.96%and 21.86%,respectively,which were lower than those in the model group(P<0.05 or P<0.01).CONCLUSION:PND showed a neuroprotective effect which may be useful for treatment of Alzheimers disease.关键词
人参环氧炔醇/网络药理学/阿尔茨海默病/神经保护Key words
panaxydol/network pharmacology/Alzheimers disease/neuroprotection分类
中医学
