癌变·畸变·突变2025，Vol.37Issue(3)：209-214,220,7.DOI:10.3969/j.issn.1004-616x.2025.03.006
胞质多聚腺苷酸结合蛋白4在肝癌中的表达及其功能
Expression and function of PABPC4 in liver cancers
摘要
Abstract
OBJECTIVE:To investigate the expression characteristics,prognostic significance and functional role of cytoplasmic polyadenylate-binding protein 4(PABPC4)in liver cancers.METHODS:RNA sequencing data of liver cancers from The Cancer Genome Atlas(TCGA)were downloaded to analyze relationships between PABPC4 expression and patient prognosis.PABPC4 knockdown and overexpression liver cancer cell lines were constructed,and expression of PABPC4,its stemness-related indexes mRNA and protein in cells were detected by real-time quantitative PCR and Western blot.Effects of PABPC4 on cell proliferation,migration,invasion and stemness were studied by CCK-8 method,plate colony formation assay,cell scratch assay and Transwell assay.RESULTS:Expression levels of PABPC4 in liver cancer tissues gradually increased with increase of pathological grade.Patients with high expression had shorter overall survival and recurrence-free survival time(P<0.05).In tumor tissues with the high expression showed upregulated PABPC4,Wnt signaling pathway-related proteins.The successfully constructed HLF cells showed stable knockdown of PABPC4 and Huh7 cells with stable overexpression.Functional experiments showed that knockdown of PABPC4 showed significantly inhibited cell proliferation,migration,invasion and colony formation(P<0.05).Overexpression of PABPC4 had the opposite effect(P<0.05).In addition,after high expression of PABPC4,the mRNA expression levels of cell stemness-related indicators(Nanog,OCT4,CD133,SOX2)were significantly increased.CONCLUSION:PABPC4 was highly expressed and was correlated with poor prognosis in liver cancer patients.PABPC4 enhanced cancer cell stemness and promoted proliferation,survival,migration,and invasion of cancer cells.Therefore,PABPC4 is a potential target for prognostic prediction and therapeutic intervention in liver cancer.关键词
肝癌/胞质多聚腺苷酸结合蛋白4/预后预测/增殖存活/迁移侵袭/细胞干性Key words
liver cancer/PABPC4/prognostic prediction/proliferation and survival/migration and invasion/cell stemness分类
临床医学引用本文复制引用
刘鑫苗,高红军,康艳,张赟,孙玉琳..胞质多聚腺苷酸结合蛋白4在肝癌中的表达及其功能[J].癌变·畸变·突变,2025,37(3):209-214,220,7.基金项目
国家自然科学基金(82073327) （82073327）
中国医学科学院医学与健康科技创新工程(2021-I2M-1-066) （2021-I2M-1-066）
