安徽农业大学学报2025，Vol.52Issue(2)：181-187,7.DOI:10.13610/j.cnki.1672-352x.20250519.004
大豆主栽品种中适宜农杆菌介导的转基因受体筛选
Screening of suitable transgenic receptors for Agrobacterium-mediated transformation in main soybean cultivars
摘要
Abstract
[Objective]Agrobacterium-mediated genetic transformation of soybean cotyledonary nodes is a key technology to achieve targeted improvement of soybean varieties,but it is susceptible to the limitations of the genetic background,and the receptor genotype is one of the key factors affecting the transformation efficiency.Therefore,this study aimed to establish a genetic transformation system for major cultivated varieties,so as to provide a foundation for achieving targeted improvement of soybean cultivars.[Method]In this research,the plant expression vector containing GUS reporter gene was used to optimize the agrobacterium-mediated genetic trans-formation system of soybean cotyledon nodes.[Result]During the co-culture period,positioning explants hori-zontally with the adaxial surface upward and supplementing the medium with 150 mg·L-1 dithiothreitol(DTT)reduced tissue browning by 15%and increased bud induction rate by approximately 15%.Application of this op-timized system to 14 elite soybean cultivars revealed distinct genotypic variations.High regeneration and trans-formation efficiencies were observed in Zhonghuang 13,Huachun 6,and Jidou 17 with the highest value of 5.30%,10.22%and 4.20%,respectively,while Heihe45 exhibited strong regeneration capacity but low transformation efficiency with the maximum transformation efficiency of 2%.Cultivars including Hefeng 55,Hefeng 50,Henong 71,Mengdou 1137,Heike 60,Henong 85,Heike 88,Henong 95,Heinong 84,and Heinong 531 demonstrated both low regeneration and transformation efficiencies(less than 2%).[Conclusion]The findings establish a foundation for achieving targeted improvement of major soybean cultivars.关键词
大豆/遗传转化/再生效率/转化率Key words
soybean/genetic transformation/regeneration efficiency/conversion rate分类
农业科学引用本文复制引用
厉晓玉,孙大千,张皓,郭诗雨,王雪晴,王琦,李英慧..大豆主栽品种中适宜农杆菌介导的转基因受体筛选[J].安徽农业大学学报,2025,52(2):181-187,7.基金项目
高产优质大豆种质创新与新品种选育项目(YZ2023005)资助. （YZ2023005）
