安徽农业大学学报2025，Vol.52Issue(2)：188-194,7.DOI:10.13610/j.cnki.1672-352x.20250519.008
蓝莓果实中GA3响应关键基因VcGA2ox2的克隆与表达分析
Cloning and expression analysis of GA3 response key gene VcGA2ox2 in blueberry fruits
摘要
Abstract
[Objective]This research aimed to provide a molecular bioinformatics basis for constructing vec-tors and functionally validating VcGA2ox2 in blueberry.[Method]Using exogenous GA3 to treat fertilized blue-berry flowers and obtain seedless mature blueberry fruits as the materials,a differentially expressed gene named VcGA2ox2 was screened using transcriptome data,and gene cloning and bioinformatics analysis were performed on it.[Result]VcGA2ox2 gene was successfully cloned and analyzed,containing an open reading frame(ORF)of 996 bp,encoding 331 amino acids with two conserved domains(2OG-FeII-Oxy and DIOX-N),belonging to a hydrophilic protein.The phylogenetic tree analysis of amino acid sequences showed that the VcGA2ox2 protein was closely related to CsGA2ox2 and CrGA2ox2 in the Camelliaceae family;qRT-PCR analysis results showed that VcGA2ox2 was expressed in blueberry flowers,leaves,stems,fruits and roots,and it had tissue specificity,with the highest expression level in the roots.GA3 treatment could up-regulated the expression of VcGA2ox2 in fruits at the two stages.[Conclusion]It was speculated that GA3 treatment can induce high expression of GA2ox2,promote GA inactivation in blueberry fruits,and inhibit ovule development,resulting in seedless blueberry fruits.关键词
无籽蓝莓/外源GA3/VcGA2ox2/分子克隆与表达Key words
seedless blueberry/exogenous GA3/VcGA2ox2/molecular cloning and expression分类
园艺学与植物营养学引用本文复制引用
王小敏,胡丽超,平心悦,吴雅琼,吴文龙,李维林..蓝莓果实中GA3响应关键基因VcGA2ox2的克隆与表达分析[J].安徽农业大学学报,2025,52(2):188-194,7.基金项目
江苏省科技项目(BE2022372)和江苏省种业振兴揭榜挂帅项目(JBGS[2021]021)共同资助. （BE2022372）
