安徽农业大学学报2025，Vol.52Issue(2)：220-227,8.DOI:10.13610/j.cnki.1672-352x.20250519.002
互叶白千层精油的提取及其抗氧化研究
Extraction and antioxidant study of essential oil from Melaleuca alternifolia(Maiden&Betche)Cheel
摘要
Abstract
[Objective]This study aimed to investigate the optimal extraction conditions of tea tree oil(TTO)by supercritical CO2 extraction and analyze its composition and antioxidant capacity.[Method]The pa-rameters of supercritical CO2 extraction were optimized by response surface method,and the components of TTO were identified by GC-MS(gas chromatography-mass spectrometry)combined with retention index(RI)method.DPPH and OH radicals scavenging test were used to analyze the antioxidant activity of TTO in vitro.The activities of SOD,GSH-Px,CAT and the level of MDA in serum and liver of D-galactose-induced aging mice were measured to analyze the antioxidant activity of TTO in vivo.[Result]The optimal supercritical CO2 extraction process of TTO was as follows:extraction pressure of 30 MPa,extraction temperature of 55℃,ex-traction time of 150 min,and the extraction yield of TTO was 2.239%under the conditions,which was basical-ly consistent with the predicted value(2.241%)of regression model in response surface optimization test.GC-MS combined with RI analysis showed that 49 components were separated from the extracted TTO,of which 16 component contents were more than 1%,and the component with the highest content was ter-pinene-4-ol.The antioxidant results in vivo and in vitro showed that TTO had a good antioxidant effect,which could effectively reduce the production of DPPH and OH free radicals,reduce the concentrations of SOD,GSH-Px and CAT,and also effectively reduce the content of MDA.[Conclusion]The supercritical CO2 extrac-tion method can efficiently extract TTO with antioxidant activity from Melaleuca alternifolia(Maiden&Betche)Cheel.关键词
互叶白千层(澳洲茶树)/超临界CO2/GC-MS/抗氧化Key words
Melaleuca alternifolia(Maiden&Betche)Cheel(Australian tea tree)/supercritical CO2/GC-MS/antioxidant分类
化学工程引用本文复制引用
刘喜明,曹红云,廖晓宝,郭丽斌,马景蕃..互叶白千层精油的提取及其抗氧化研究[J].安徽农业大学学报,2025,52(2):220-227,8.基金项目
福建省科技厅对外合作项目(2024I0025)和福建省自然科学基金面上项目(2018J01458)共同资助. （2024I0025）
