中医康复2025，Vol.2Issue(7)：42-47,6.DOI:10.19787/j.issn.2097-3128.2025.07.007
火针镇痛外周敏化机制初探及对带状疱疹后神经痛大鼠背根神经节中神经损伤诱导蛋白(NINJ1,NINJ2)、疼痛介质的影响
Exploration of Peripheral Sensitization Mechanisms of Fire Needling Analgesia and Its Effects on Nerve Injury-Induced Proteins(NINJ1,NINJ2)and Pain Mediators in Dorsal Root Ganglia of Rats with Postherpetic Neuralgia
摘要
Abstract
Objective:To observe the pain behavioral changes in rats with postherpetic neuralgia(PHN)treated with fire needle therapy,as well as changes in the expression of nerve injury-induced proteins 1,2(Ninjurin1,NINJ1;Ninjurin2,NINJ2)and pain me-diators in the dorsal root ganglia(DRG),to explore the analgesic mechanism of fire needle therapy.Methods:45 rats were randomly divided into a blank group,a model group,a solvent group(sham model),a fire needle group,and a gabapentin group,with 9 rats in each group.PHN rat models were prepared by intraperitoneal injection of resin toxin(RTX);After successful modeling,the fire needle group and gabapentin group were given corresponding intervention measures.After treatment,Western blotting was used to detect the expression levels of substance P(SP),calcitonin gene-related peptide(CGRP),glutamate(Glu),and NINJ1 and NINJ2 in the DRG of the 4th to 6th lumbar vertebrae of each group of rats,in order to explore the analgesic effect and mechanism of fire needle on PHN rats.Results:RTX intraperitoneal injection successfully established PHN models.After fire needle intervention,the mechanical pain threshold of PHN rats increased and the thermal pain threshold decreased,showing a continuous trend of change,in-dicating that fire needle has a significant analgesic effect;Under the intervention of fire needle,SP,CGRP,Glu,NINJ1,and NINJ2 in the dorsal root ganglia of rats were significantly reduced compared to the RTX group,indicating that the peripheral sensitization process of neuropathic pain was intervened.Conclusion:Fire needle therapy for PHN may regulate pain mediators and NINJ1 and NINJ2,intervene in the peripheral sensitization process to exert analgesic effects.关键词
带状疱疹后神经痛/火针/神经损伤诱导蛋白1/神经损伤诱导蛋白2/P物质(SP)/降钙素基因相关肽(CGRP)/谷氨酸(Glu)/外周敏化/镇痛效应Key words
postherpetic neuralgia/fire needling/nerve injury-induced protein 1/nerve injury-induced protein 2/substance P(SP)/calcitonin gene-related peptide(CGRP)/glutamate(Glu)/peripheral sensitization/analgesic effect分类
中医学引用本文复制引用
刘琨,韦永政,曾婧纯,林诗雨,王澍欣,邝伟川,王鑫栋,朱瑜,刘悦..火针镇痛外周敏化机制初探及对带状疱疹后神经痛大鼠背根神经节中神经损伤诱导蛋白(NINJ1,NINJ2)、疼痛介质的影响[J].中医康复,2025,2(7):42-47,6.基金项目
广州市科技局基础与应用基础研究项目(202201011279) （202201011279）
广东省基础与应用基础研究基金委员会区域联合基金-青年基金项目(2021A1515110736) （2021A1515110736）
