长春中医药大学学报2025，Vol.41Issue(6)：634-639,6.DOI:10.13463/j.cnki.cczyy.2025.06.010
太白贝母调控PI3K/AKT通路抑制肝癌细胞增殖与迁移
Study on Taipei Fritillary Bulb on the inhibition of proliferation and migration of liver cancer cell through PI3K/AKT signaling pathway
摘要
Abstract
Objective To investigate the effects of Taipei Fritillary Bulb(TFB)on the proliferation and migration of hepatocellular carcinoma HepG2 and Bel-7402 cells and its mechanism.Methods 50 SD rats were randomly divided into the blank control group,TFB low(0.5 g·mL-1)group,TFB medium(1 g·mL-1)group,TFB high(2 g·mL-1)group and cisplatin positive control group(1 mg·mL-1).Drug-containing serum was prepared after 7 days of intragastric administration for the culture of HepG2 and Bel-7402 cells.Cell proliferation ability was detected by CCK-8 assay.Cell migration ability was detected by scratch test.Western Blot assay was used to detect expression levels of cysteine aspartic protease Cleaved Caspase-3 and Cleaved Caspase-9,Blymphocyte tumor(Bcl)-2 related proteins Bax,Bcl-2,PI3K,AKT,human phosphorylated p-PI3K,and p-AKT protein;The relative expression levels of PI3K and AKT mRNA were detected by RT-qPCR.Results TFB might inhibit proliferation and migration of HepG2 and Bel-7402 cells(P<0.05,P<0.01),increase protein expression of Bax,Cleaved Caspase-3,Cleaved Caspase-9,and decrease the expression of Bcl-2 and the relative expression of PI3K and AKT mRNA(P<0.05,P<0.01).Conclusion TFB can inhibit the proliferation and migration of hepatocellular carcinoma cells by regulating PI3K/AKT signaling pathway.关键词
太白贝母/肝癌/PI3K/AKT信号通路/增殖/迁移Key words
Taipei Fritillary Bulb/liver cancer/PI3K/AKT signaling pathway/proliferation/migration分类
医药卫生引用本文复制引用
张睿,任清维,卫培峰,李媛媛,晁旭..太白贝母调控PI3K/AKT通路抑制肝癌细胞增殖与迁移[J].长春中医药大学学报,2025,41(6):634-639,6.基金项目
陕西省中医药管理局科研课题(2021-ZZ-JC001) （2021-ZZ-JC001）
陕西省中医药"双链融合"中青年科研创新团队建设项目(2022-SLRH-LJ-010) （2022-SLRH-LJ-010）
咸阳市中西医结合防治肿瘤重点实验室项目(L2023-CXNL-CXPT-ZDSYS-002) （L2023-CXNL-CXPT-ZDSYS-002）
