高原农业2025，Vol.9Issue(3)：277-293,17.DOI:10.19707/j.cnki.jpa.2025.03.001
饲料中添加不同浓度百合珠芽对西藏藏猪卵巢卵泡发育及LncRNA表达的影响
Effects of Adding Different Concentrations of The Bulbils of Lilium Lancifolium Supplementation to Feed on Follicle Development and LncRNA Expression in the Ovary of Xizang Pigs
摘要
Abstract
This study aimed to reveal the effects of different concentrations of the bulbils of Lilium lancifolium in the basic diet on the development of ovarian follicles and the expression of related lncrnas in Xizang pigs.Xizang pigs were used as the research object,and the same batch of nine healthy Xizang pigs with a weight of(17.40±1.15)kg was randomly divided into three groups,of which the BW group was the control group,fed on the basal diet,the BY group and the BR group were the experimental groups,with 1%concentration lily bead buds and 2%concentration lily bead buds added to the basal diet,respectively,and after 6 weeks of experimental feeding,morphological observation,ovarian organ index measurement,and statistics of the number of different types of follicles in the ovaries were carried out to judge the ovarian development.The high-throughput omics transcriptome technology was used to sequence the ovarian LncRNA,and the differential LncRNA was verified in time quantitatively to verify the reliability of the transcriptome sequencing results.The results showed that Ovarian surface and follicle characteristics:In the BR group,large follicles appeared on the ovarian surface of Xizang pigs;there was no significant difference in the ovarian index among the groups.Follicle ratio analysis revealed that the BW group had the highest proportion of primordial follicles,the BY group had the highest proportion of secondary follicles,and the BR group had the highest proportion of mature follicles.LncRNA screening and expression revealed 5,772 new lncRNAs.Expression analysis revealed that the BY group displayed the highest average expression level,whereas the BW group had the lowest expression level,and the three groups had similar expression trends,with strong correlations among the BY,BW,and BR groups.Differential expression analysis revealed that the BW vs.BY comparison identified 2,021 differentially expressed lncRNA genes(1,197 upregulated and 824 downregulated in the BY group compared with the BW group);the BW vs.BR comparison identified 1,308 differentially expressed lncRNA genes(829 upregulated and 479 downregulated in the BR group compared with the BW group);the BY vs.BR comparison screened 1,330 differentially expressed lncRNA genes(646 upregulated and 684 downregulated in the BR group compared with the BY group);and LncRNA target gene prediction and functional analysis:Target gene prediction identified CCNL2,MAP3K14,STUB1,IQANK1,and PKP3.GO and KEGG enrichment analyses of target genes revealed significant enrichment of cAMP,MAPK,PI3K-Akt,Wnt,and other ovarian development signaling pathways.The genes related to these pathways included LOC110261342,MSTRG.26467,FXN,CLP1,Gem,PODNL1,and TGFBR3L.The results showed that the addition of Lilium bulbiferous bulbs to the diet,especially at a 2%concentration,promoted the development of mature ovarian follicles in Xizang pigs and significantly regulated the expression of long noncoding RNAs(lncRNAs),which were involved in multiple biological pathways related to ovarian development.These results indicated that this study would provide fundamental data and theoretical support for further understanding the enhancement of Xizang pig reproductive capacity by Lilium bulbiferous bulbs.关键词
lncRNA/卷丹百合珠芽/西藏藏猪/靶基因Key words
Long Noncoding RNA/Lilium bulbiferous bulbs/Xizang pigs/Target genes分类
农业科技引用本文复制引用
任子利,曹婷婷,阚勇,王雨佳,赵彦玲..饲料中添加不同浓度百合珠芽对西藏藏猪卵巢卵泡发育及LncRNA表达的影响[J].高原农业,2025,9(3):277-293,17.基金项目
国家自然科学基金资助项目[藏猪精子高原适应性的DNA甲基化机制(31760669)] （31760669）
藏猪睾丸高原适应性的DNA甲基化机制(31660658) （31660658）
西藏自治区科技厅重点研发计划项目[藏羚羊高原适应的特殊遗传机制研究(XZ202402ZY0025)]. （XZ202402ZY0025）
