高原农业2025，Vol.9Issue(3)：304-310,333,8.DOI:10.19707/j.cnki.jpa.2025.03.003
基于TTC法对急尖长苞冷杉种子生活力分析
Analysis of Seed Viability of Abies georgei var.Smithii Based on TTC Method
摘要
Abstract
The protection level of Abies georgei var.smithii acuminata in China is Grade II.,which is highly sensitive to the environment.At present,the population of Abies georgei var.smithii has a decreasing trend,and seed reproduction is the main way of its natural regeneration.Therefore,its seed viability is crucial to the continuation of the population.In order to detect seed viability more scientifically and efficiently,the TTC method was used to detect seed viability,and the optimal combination of 5 factors including soaking temperature,soaking time,TTC concentration,dyeing temperature and dyeing time was selected through orthogonal experimental design.The results showed that:(1)The TTC method was feasible to detect the viability of Abies georgei var.smithii seeds.Compared with the germination test,the TTC method could save more time and cost in seed quality judgment;(2)The effects of various factors on the viability of Abies georgei var.smithii seeds were as follows:TTC concentration>soaking temperature>dyeing temperature>soaking time>dyeing time;(3)The optimum conditions for the determination of seed viability by TTC were as follows:after soaking seeds at 20℃for 12 h,the dye was stained with 0.3%TTC solution at 30℃for 12 h in the dark.关键词
急尖长苞冷杉种子/TTC法/生活力/色季拉山Key words
Abies georgei var.smithii seeds/TTC Method/Viability/Sejira Mountain分类
农业科技引用本文复制引用
赵迎霞,任毅华,普布扎西..基于TTC法对急尖长苞冷杉种子生活力分析[J].高原农业,2025,9(3):304-310,333,8.基金项目
国家自然科学基金资助项目(32371651) （32371651）
西藏高原森林生态教育部重点试验室研究生创新计划项目(XZA-JYBSYS-2023-21). （XZA-JYBSYS-2023-21）
