海南医科大学学报2025，Vol.31Issue(11)：801-807,7.DOI:10.13210/j.cnki.jhmu.20240722.001
基于PGC-1α/PPARα信号通路探讨稳心颗粒对心梗后心衰大鼠能量代谢的影响
Exploring the effects of Wenxin granules on energy metabolism in rats with post-myocardial infarction heart failure based on the PGC-1α/PPARα signaling pathway
摘要
Abstract
Objective:To investigate how Wenxin granules regulate energy metabolism in post-myocardial infarction(MI)heart failure rats via the peroxisome proliferator-activated receptor gamma coactivator 1-alpha(PGC-1α)/peroxisome proliferator-activated receptor alpha(PPARα)signaling pathways.Methods:The rat model of post-myocardial infarction heart failure was es-tablished by ligating the left anterior descending coronary artery.Subsequently,the rats were randomly categorized into the sham operation group,model group,low-dose Wenxin Granules group,high-dose Wenxin Granules group,and trimetazidine group.Af-ter 4 weeks,cardiac function was assessed by echocardiography,and cardiac troponin T(cTnT)levels were measured using ELI-SA.ATP,AMP,and ADP levels were determined,and ADP/ATP and AMP/ATP ratios calculated.Mitochondrial respiratory chain complexⅠ、Ⅱ、Ⅳ、Ⅴ activities were evaluated.Western blotting was used to assess PGC-1α,PPARα,carnitine palmitoyl-transferase 1α(CPT1α)and fatty acid transport protein(CD36).Results:Compared with the sham operation group,the cardiac function parameters of the model group significantly decreased.The structure-related parameters showed pathological characteris-tics of thinner ventricular walls and enlarged heart cavities.The level of cTnT was significantly increased,along with reduced re-spiratory chain complex activities and ATP levels,and increased AMP and ADP levels.Protein expressions of PGC-1α,PPARα,CD36,and CPT1α were significantly downregulated.Compared with the model group,the above detection indexes of the Wenxin granules group showed varying degrees of improvement after intervention.Conclusion:Wenxin granules,by modulating the PGC-1α/PPARα signaling pathway,enhance energy metabolism and confer cardioprotection on rats with post-myocardial infarction heart failure.关键词
稳心颗粒/线粒体/心梗后心衰/能量代谢Key words
Wenxin granules/Mitochondria/Post-infarction heart failure/Energy metabolism分类
中医学引用本文复制引用
崔喜元,杨丁,王哲,娄利霞,聂波,赵久丽,吴爱明..基于PGC-1α/PPARα信号通路探讨稳心颗粒对心梗后心衰大鼠能量代谢的影响[J].海南医科大学学报,2025,31(11):801-807,7.基金项目
This study was supported by General Project of National Natural Science Foundation of China(82274449) （82274449）
Beijing University of Traditional Chinese Medicine Dongzhimen Hospital 2022 Science and Technology Innovation Project(DZMKJCX-2022-008) 国家自然科学基金面上项目(82274449) （DZMKJCX-2022-008）
北京中医药大学东直门医院2022科技创新专项(DZMKJCX-2022-008) （DZMKJCX-2022-008）
