干旱区研究2025，Vol.42Issue(6)：1055-1066,12.DOI:10.13866/j.azr.2025.06.09
呼伦贝尔沙地樟子松林土壤和根内真菌泛化种和特化种结构与功能特征
Structure and functional group characteristics of generalized and specialized species of soil and root-associated fungi in Pinus sylvestris var.mongolica forests of the Hulunbuir Desert
摘要
Abstract
This study investigated the community structure and function of generalized and specialized fungal species(GFS and SFS,respectively)in the soil and roots of Pinus sylvestris var.mongolica forests and their rela-tionship with soil physicochemical properties,aiming to identify key microbial mechanisms affecting ecosystem functioning in P.sylvestris var.mongolica forests.Using high-throughput sequencing technology,we analyzed the differences in the community structure and functional groups of GFS and SFS in the soil and roots of natural forests and plantations(24 a,35 a,44 a)of P.sylvestris var.mongolica in the Hulunbuir Desert.The results were as follows:(1)The soil and root-associated GFS retained 169 operational taxonomic units(OTUs)in total,the soil SFS retained 603 OTUs,and the root-associated SFS retained 216 OTUs,including Tricholoma and Suillus in soil GFS;and Tricholoma,Suillus,and Cadophora in root-associated GFS;Penicillium in soil SFS;and Aceph-ala in root-associated SFS.(2)The relative abundance of symbiotic nutritive fungi accounted for 28.49%-47.21%of soil GFS,and the dominant ecological functional group was ectomycorrhizal fungi,which showed a trend of increasing and then decreasing with forest age.Saprophytic nutritive fungi accounted for 17.01%-40.01%of soil SFS.The relative abundance of saprophytic nutritive fungi in plantation forests was lower than that in natural forests,and it showed a tendency of increasing and decreasing followed by increasing with forest age.Symbiotic trophic fungi accounted for 43.25%-54.45%of the root-associated GFS;the dominant ecological functional group was ectomycorrhizal fungi,which showed an increasing trend with increasing forest age,and the relative abundance of ectomycorrhizal fungi in natural forests was higher than that in plantation forests.(3)The soil organic matter and available phosphorus content of natural P.sylvestris var.mongolica forests were signifi-cantly higher than those of plantation forests(P<0.05).In plantation forests,with increasing forest age,the soil or-ganic matter(SOM),total phosphorus(TP),available nitrogen(AN),and available phosphorus in the soil in-creased significantly(P<0.05),whereas the soil pH decreased but not significantly(P>0.05).GFS was mainly dominated by SOM,soil NH4+-N,and TP(P<0.05).Furthermore,the community variability of root-associated SFS was regulated by total nitrogen(P<0.05),whereas soil SFS was significantly affected by only TP,AN,and NO3--N(P<0.05).The drivers of fungal community structure showed significant ecological niche differentiation.This study contributes to a deeper understanding of the ecological functions of fungi in the soil and roots of P.syl-vestris var.mongolica forests,providing a basis for the sustainable management and protection of P.sylvestris var.mongolicaforests in the Hulunbuir Desert.关键词
土壤真菌/根内真菌/泛化种/特化种/群落结构/生态功能/土壤理化性质Key words
soil fungi/root-associated fungi/habitat generalist/habitat specialist/community structure/ecolog-ical function/soil physical and chemical引用本文复制引用
成艳琳,王家源,高广磊,丁国栋,张英,赵珮杉,朱宾宾..呼伦贝尔沙地樟子松林土壤和根内真菌泛化种和特化种结构与功能特征[J].干旱区研究,2025,42(6):1055-1066,12.基金项目
国家自然科学基金项目(32371962) （32371962）
