海南医科大学学报2025，Vol.31Issue(11)：816-823,8.DOI:10.13210/j.cnki.jhmu.20240914.003
有氧运动抑制炎症反应改善动脉粥样硬化小鼠心肌细胞凋亡的机制研究
Aerobic exercise inhibits inflammatory response to improve apoptosis in atherosclerotic mice cardiomyocytes
摘要
Abstract
Objective:To investigate the effects and mechanisms of aerobic exercise to inhibit inflammatory response on cardio-myocyte apoptosis in atherosclerotic mice.Methods:Male ApoE-/-knockout mice were selected at 8 weeks of age,and after 1 week of adaptive feeding,they were randomly divided into a control group,a model group and an aerobic exercise group,with 9 mice in each group;model group and aerobic exercise group were fed with"Western-type"dietary feeds for 12 weeks to establish an atherosclerosis model,while the aerobic exercise group underwent aerobic training at the same time.After 12 weeks,the cardio-myocyte apoptosis was detected in mice by TUNEL staining.The expression of NOD-like receptor protein 3(NLRP3),interleu-kin-1β(IL-1β)and transforming growth factor-β1(TGF-β1)were detected by immunohistochemistry in each group.The protein levels of NLRP3,IL-1β and TGF-β1 were detected by Western blot.The kit detects the activity of lactate dehydrogenase(LDH)and creatine kinase(CK)in mice serum,and ELISA detects the level of cardiac troponin I(cTnⅠ)in serum.Results:TUNEL staining showed that there were statistically differences in cardiomyocyte apoptosis among the three groups(P<0.01);mean-while,immunohistochemistry showed that the expression of NLRP3,IL-1β and TGF-β1 protein in myocardial tissue of the model group was higher than that of the control group and the aerobic exercise group(P<0.05).Western blot showed that the levels of NLRP3,IL-1β and TGF-β1 protein in the myocardial tissue of the model group and the aerobic exercise group were significantly higher than those in the control group(P<0.01).Compared with the model group,the levels of NLRP3,IL-1β and TGF-β1 pro-tein in the myocardial tissue of the aerobic exercise group were significantly decreased(P<0.001).The differences between the three groups in LDH,CK and cTnⅠ were statistically significant(P<0.01).Conclusion:Aerobic exercise can improve cardio-myocyte apoptosis in atherosclerotic mice,and its mechanism may be related to the inhibition of inflammatory response in myocar-dial tissue and the reduction of myocardial enzyme.关键词
有氧运动/动脉粥样硬化/心肌酶/炎症/凋亡Key words
Aerobic exercise/Atherosclerosis/Cardiac enzymes/Inflammation/Apoptosis分类
临床医学引用本文复制引用
马甜甜,秦芳,于子夫,刘西花..有氧运动抑制炎症反应改善动脉粥样硬化小鼠心肌细胞凋亡的机制研究[J].海南医科大学学报,2025,31(11):816-823,8.基金项目
This study was supported by the National Natural Science Foundation of China(81802239) 国家自然科学基金资助项目(81802239) （81802239）
