高原农业2025，Vol.9Issue(3)：362-370,9.DOI:10.19707/j.cnki.jpa.2025.03.009
基于综合生物信息学分析拟南芥耐寒核心差异表达基因
Ananlysis of Arabidopsis Thaliana's Coldtolerant Core Differentially Expressed Genes Based on Comprehensive Bioinformatics
摘要
Abstract
The objective of this study was to screen the differentially expressed genes and related metabolic pathways in the core of Arabidopsis thaliana under low temperature stress.The chip dataset related to low temperature stress in Arabidopsis thaliana was retrieved in the GEO database,and the GEO2R tool in GEO was used to screen the differentially expressed genes,and then the GO enrichment and KEGG enrichment annotations were performed on the selected differentially expressed genes by using the DAVID database,and then the protein-protein interaction network diagram was constructed by combining the STRING database with the differentially expressed genes.The results showed that 788 differentially expressed genes were screened out by identifying GSM2844128,GSM2844129,GSM2844132 and GSM2844133 samples in the chip GSE106635,of which 491 were up-regulated and 297 were down-regulated.DAVID online functional enrichment analysis showed that low temperature stress had a significant effect on protein kinase activity and protein binding in Arabidopsis.The core differentially expressed genes that played a key role in low temperature stress,such as CCA1,LHY,LTI78,COR15A,SUS1,COR47,PRR5,GI,ABA1 and LEA14,were excavated.关键词
低温胁迫/GEO数据库/拟南芥/生物信息学Key words
Low temperature stress/GEO database/Arabidopsis thaliana/Bioinformatics分类
农业科技引用本文复制引用
张军,李伟,王若丁,高海娟,李旭业..基于综合生物信息学分析拟南芥耐寒核心差异表达基因[J].高原农业,2025,9(3):362-370,9.基金项目
黑龙江省省属科研院所科研业务费项目(CZKYF2023-1-B002). （CZKYF2023-1-B002）
