贵州农业科学2025，Vol.53Issue(5)：16-23,8.DOI:10.3969/j.issn.1001-3601.2025.05.003
低温胁迫不同地区铁皮石斛的生理响应及其抗寒性评价
Physiological Response of Dendrobium officinale from Different Regions Under Low Temperature Stress and Evaluation on Its Cold Resistance
摘要
Abstract
[Objective]The physiological index change of Dendrobium officinale from different regions under low temperature stress was explored,and the key indicators influencing cold resistance were identified,which provided references for promoting its cultivation in suitable regions.[Method]D.officinale samples from different regions were subjected to low temperature treatments at 4℃,0℃,-4℃,and-8℃.Physiological traits including soluble protein(SP),malondialdehyde(MDA),chlorophyll(Chl)content,and activities of three antioxidant enzymes[superoxide dismutase(SOD),peroxidase(POD),and catalase(CAT)]were measured.The LT50 was evaluated by fitting relative electrical conductivity(REC)under different temperatures.Variance analysis,correlation analysis,and principal component analysis(PCA)were conducted to study cold resistance mechanisms.[Result]LT50 of D.officinale from different regions followed GZY-K(-0.484 6℃)＞GZY-080(-0.516 3℃)＞GZY-003(-2.241 0℃)＞GZY-Y(-3.299 1℃),with GZY-Y exhibiting the strongest cold resistance.At-4℃,severe frost damage occurred,and the cold resistance system collapsed.Extremely significant differences in physiological index of D.officinale from different regions were observed among cultivars and temperature treatments,indicating rich genetic diversity in cold resistance.Under low temperature stress,physiological index existed complex correlation.CAT activity showed a highly significant negative correlation with SOD activity and EC,but a significant positive correlation with POD activity.MDA content was highly and negatively correlated with POD activity and significantly and negatively correlated with Chl content.Chl content was highly and positively correlated with SP.SPs was highly and negatively correlated with POD and SOD activities,and significantly and negatively correlated with EC.At 0℃,SOD activity,Chl content,SP,and POD activity synergistically or antagonistically determined cold resistance.[Conclusion]Cold resistance of D.officinale from different regions follows GZY-Y＞GZY-003＞GZY-080＞GZY-K,with GZY-Y being the most cold-tolerant,suitable for breeding cold-resistant cultivars.Cold resistance mechanisms vary significantly among regional D.officinale under low temperature stress.SOD activity,Chl content,SP,and POD activity are critical traits for cold resistance formation of D.officinale.关键词
铁皮石斛/抗寒性/LT50评价法/生理生化/抗氧化酶/种质创新Key words
Dendrobium officinale/cold resistance/LT50 evaluation method/physiological and biochemical/antio-xidant enzyme/germplasm innovation分类
农业科技引用本文复制引用
任得强,张赛,李钰娇,陈春伶..低温胁迫不同地区铁皮石斛的生理响应及其抗寒性评价[J].贵州农业科学,2025,53(5):16-23,8.基金项目
贵州省科技厅基础研究计划项目"道地苗药红花龙胆良种选育及种子种苗标准化繁育技术研究"(黔科合基础-ZK[2022]一般481) （黔科合基础-ZK[2022]一般481）
贵州中医药大学博士启动资金项目"铁皮石斛全基因组SSR分子标记的开发及种质资源遗传多样性分析"(贵中医博士启动[2019]67号),"贵州(黔产)石斛属药用植物种质资源搜集及遗传多样性分析"(贵中医博士启动[2019]12号) （贵中医博士启动[2019]67号）
