福建农业学报2025，Vol.40Issue(3)：219-224,6.DOI:10.19303/j.issn.1008-0384.2025.03.001
苦瓜枯萎病抗性遗传规律分析
Genetic Analysis on Resistance of Bitter Gourd to Fusarium Wilt
摘要
Abstract
[Objective]Genetics of bitter gourd in resisting Fusarium wilt was analyzed to better understand the most effective way of the disease control by breeding wilt-resistant variety.[Method]A population of P1,P2,F1,and F2 generations was established by crossing a highly resistant Mc176 as the male parent and a susceptible Mc131 as the female parent.The seeds from the plants were sown,and the seedlings at two-leaves-and-one-bud stage inoculated with Fusarium oxysporum Schl.f.sp.momordicae by root-grafting.Twenty-five days afterward,phenotypic identification was performed on the plants.The SEA package in the R-4.1.1 software was used to analyze 24 mixed major gene+polygene models to select the best fit one for predicting bitter gourd resistance to Fusarium wilt.[Result]The 1MG-AD model was found to satisfactorily represent the wilt resistance.The additive and dominant effect values generated by the model were 2.3479 and 0.6467,respectively,with 30.86%heritability on a pair of the major genes in F2 population.[Conclusion]The Fusarium wilt resistance of bitter gourd demonstrated a significant quantitative characteristic.In the Mc131×Mc176 hybrid combination,the resistance was regulated by a pair of mainly additive genes that controlled the trait.Since the environmental factors affected more significantly than genetics on the heritability of the major genes in F2 generation population,further experiments taking external conditions into consideration are in order.关键词
苦瓜/枯萎病/遗传分析/主基因+多基因混合模型Key words
Momordica charantia L./Fusarium wilt/genetic analysis/major gene+polygene mixed model分类
园艺学与植物营养学引用本文复制引用
刘雅岚,牛玉,于仁波,易林,刘昭华,张俊红,杨衍,韩旭..苦瓜枯萎病抗性遗传规律分析[J].福建农业学报,2025,40(3):219-224,6.基金项目
海南省重点研发项目(ZDYF2024XDNY238) （ZDYF2024XDNY238）
热带作物生物育种全国重点实验室科研项目(NKLTCBCXTD35) （NKLTCBCXTD35）
海南省重大科技计划项目(ZDKJ2021010) （ZDKJ2021010）
海南省冬季瓜菜产业技术体系首席科学家项目(HNARS-05-SX) （HNARS-05-SX）
海南省冬季瓜菜产业技术体系遗传育种岗岗位专家项目(HNARS-05-G02) （HNARS-05-G02）
