广州医药2025，Vol.56Issue(5)：605-615,11.DOI:10.20223/j.cnki.1000-8535.2025.05.005
重楼皂苷Ⅰ通过p53信号通路诱导慢性粒细胞白血病细胞铁死亡
Ferroptosis of chronic myeloid leukemia cells induced by polyphyllin Ⅰ through p53 signal pathway
陈冠君 1李建厂 1孙维梅 1周明轩 1尼坤洋 1王浩1
作者信息
- 1. 滨州医学院附属医院儿童血液与内分泌科(山东 滨州 256600)
- 折叠
摘要
Abstract
Objective To investigate the inhibitory effect of polyphyllin I(PPI)on chronic myeloid leukemia cells(K562)and its possible mechanism.Methods K562 cell line was cultured in suitable environment,and the optimal concentration of the drug was screened by CCK-8 method.The optimal concentration of the drug cultured for 24 hours was used as the intervention concentration of the follow-up experiment.Cells were divided into the following groups:(1)blank group,(2)saponins group,(3)inhibitor group and(4)saponins+inhibitor group.The cell proliferation rate was detected by CCK-8 method.The cell morphology was observed by AO/EB staining.The apoptosis rate was detected by flow cytometry.The contents of reactive oxygen species(ROS),glutathione(GSH)and ferrous(Fe2+)in different groups were detected,and the expression of mRNA and protein in different groups were detected by qRT-PCR and Western blot respectively.Results PPI significantly inhibited the growth of K562 cells in a dose-and time-dependent manner.Compared with the blank group,PPI significantly inhibited the proliferation of K562 cells and increased the apoptosis rate of K562 cells,while the use of ferroptosis inhibitor(Ferrostian-1)reversed the promoting effect of PPI on apoptosis of K562 cells.Compared with the blank group,the contents of reactive oxygen species(ROS)and ferrous iron(Fe2+)increased and the content of glutathione(GSH)decreased in the saponins group.The use of Ferrostian-1 could down-regulate the contents of ROS and Fe2+and increase the content of GSH in the cells treated with the drug.Compared with the blank group,the expression of p53 mRNA and protein in the saponins group increased,while the expression of SLC7A11,GPX4 mRNA and protein decreased.The expression of p53 mRNA and protein in the saponins+inhibitor group was lower than that in the saponins group,while the expression levels of SLC7A11,GPX4 mRNA and protein increased.Conclusions PPI can effectively inhibit the proliferation of K562 cells and promote ferroptosis in K562 cells.The molecular mechanism can be related to the regulation of p53 signal pathway.关键词
重楼皂苷Ⅰ/p53信号通路/铁死亡/K562细胞Key words
polyphyllin I/p53 signaling pathways/ferroptosis/K562 cells引用本文复制引用
陈冠君,李建厂,孙维梅,周明轩,尼坤洋,王浩..重楼皂苷Ⅰ通过p53信号通路诱导慢性粒细胞白血病细胞铁死亡[J].广州医药,2025,56(5):605-615,11.
