广州医药2025，Vol.56Issue(5)：644-649,661,7.DOI:10.20223/j.cnki.1000-8535.2025.05.010
NXT629调控脂质代谢改善肝胆结石的机制研究
The mechanism of NXT629 regulating lipid metabolism to improve hepatolithiasis
摘要
Abstract
Objective To explore the mechanism on NXT629 improves hepatolithiasis formation.Methods C57BL/6J mice were fed either a regular diet or a lithogenic diet(LD),with the LD group receiving injections of PPAR-α inhibitor NXT629.Liver steatosis was analyzed via HE staining,hepatic lipid accumulation was detected by Oil Red O staining,and total cholesterol,triglycerides,phospholipids,total bile acids,cholesterol saturation index,low density lipoprotein cholesterol,and high density lipoprotein cholesterol levels in bile or serum were measured using assay kits.RT-qPCR was employed to determine the mRNA expression of ABCG5/8,CYP7A1,CYP7B1,PPAR-α,and ABCB11 in mouse liver tissues.Results The results showed that NXT629 target PPAR-α to down-regulate the mRNA levels of ABCG5,ABCG8,and ABCB11 in the livers of LD-fed mice,while increasing the mRNA levels of CYP7A1 and CYP7B1,thereby reducing LD-induced hepatolithiasis formation and improving lipid metabolism disorders.Conclusions NXT629 can improve cholesterol gallstones by affecting the expression of genes related to lipid metabolism.关键词
NXT629/脂代谢/肝胆结石/成石饮食/PPAR-αKey words
NXT629/lipid metabolism/hepatolithiasis/lithogenic diet/PPAR-α引用本文复制引用
陈浩,林梁,邹来宾,邱旭彬..NXT629调控脂质代谢改善肝胆结石的机制研究[J].广州医药,2025,56(5):644-649,661,7.基金项目
广州市花都区人民医院重点学科自拟科研项目(23ZDXKZN11) （23ZDXKZN11）
广州市花都区人民医院院内医学重点学科建设项目(消化病学科YNZDXK202201) （消化病学科YNZDXK202201）
广州市花都区人民医院院内基金项目(2021B02) （2021B02）
