河南农业大学学报2025，Vol.59Issue(3)：379-388,10.DOI:10.16445/j.cnki.1000-2340.20240924.001
RecET重组机制及应用研究进展
Research development of RecET recombination
摘要
Abstract
Based on the mechanism by which RecE/RecT(RecET)mediates short homology arms(30-60 bp)DNA recombination,this paper explores the application prospects of RecET recombination as a new tool widely used in plasmid construction and genome editing of metabolic pathway.The develop-ment of the recombination tool from construction to perfection,its application in plasmid construction and genome engineering,crystal and cryo-electron microscopy structure of the RecE/RecT proteins,and mechanism hypothesis of exonuclease activity and annealing of ssDNA were reviewed.Further-more,the existing problems and future development of in vitro recombination,in vitro interaction,and research value in eukaryotes were prospected.关键词
RecET重组/RecET基因组编辑工具/RecET蛋白结构/外切活性重组说/ssDNA退火机制Key words
RecET recombination/RecET-genome engineering tool/structure of RecET protein/recombination hypothesis of exonuclease/annealing mechanism of ssDNA分类
生物工程引用本文复制引用
郑园霞,张毅,刘亮伟..RecET重组机制及应用研究进展[J].河南农业大学学报,2025,59(3):379-388,10.基金项目
国家自然科学基金面上项目(31771915) （31771915）
