河南农业大学学报2025，Vol.59Issue(3)：455-462,8.DOI:10.16445/j.cnki.1000-2340.20241023.001
烟草源高产蛋白酶菌株筛选及其酶特性
Screening of high-yield protease strains from tobacco and its enzyme characteristics
摘要
Abstract
[Objective]This study aimed to screen microbial strains capable of efficiently degrading tobacco leaf proteins,study their enzymatic properties of proteases,and explore proteases with indus-trial application value.[Method]The strains with high protein degradation efficiency were isolated from the surface of tobacco leaves by protease selective medium and enzyme activity determination.The morphological,molecular biology,physiological and biochemical identification of the screened strains were carried out,and the characteristics of the protease produced(temperature,pH value,metal ion)were further studied to explore its effect on protein degradation of tobacco leaves.[Result]A bacterial strain YANDB-21 with high efficiency protein degradation was screened and identified as Stenotrophomonas maltophilia.The protease produced by the bacteria has enzyme activity under acidic,neutral and alkaline conditions.The optimum reaction temperature of the protease was 50℃,and the optimum reaction pH was 10.0.The enzyme has good temperature tolerance below 40℃,and good stability in the pH 3.0～12.0.The metal ions K+,Cu2+,Ca2+and Mn2+can improve the enzyme activity,while Mg2+,Zn2+,Fe2+and Fe3+inhibit the enzyme activity.The crude enzyme solution was used to treat Shaanxi Shangluo tobacco leaves,and the protein degradation rate was 22.42%.[Conclu-sion]The protease enzyme produced by strain YANDB-21 has high activity and good stability,and can efficiently degrade tobacco leaf protein,which is suitable for promotion and application in tobacco pro-cessing and production.关键词
蛋白质降解菌/嗜麦芽寡养单胞菌/烟叶/蛋白酶/酶学性质/耐受性Key words
protein degrading bacteria/Stenotrophomonas maltophilia/tobacco leaf/protease/enzy-matic properties/tolerance分类
生物科学引用本文复制引用
张有奇,张泉彬,霍天辰,李满,王欢欢,武云杰,付博,郭志刚,寇莉青..烟草源高产蛋白酶菌株筛选及其酶特性[J].河南农业大学学报,2025,59(3):455-462,8.基金项目
河南省科技研发计划联合基金(应用攻关类)项目(242103810005) （应用攻关类）
陕西中烟工业有限责任公司科技项目(BA000-ZB23007) （BA000-ZB23007）
河南省研究生教育改革与质量提升工程项目(YJS2024JD17) （YJS2024JD17）
