农业科技与技术（英文）2025，Vol.26Issue(1)：45-54,10.DOI:10.16175/j.cnki.1009-4229.2025.01.006
甘蓝型油菜BnZAT12基因的克隆及表达载体构建
Cloning and Expression Vector Construction of BnZAT12 in Brassica napus
摘要
Abstract
To investigate the function of the zinc finger protein BnZAT12 in Brassica napus,bioinformatics analysis was conducted on BnZAT12.The results showed that the open reading frame of BnZAT12 was 477 bp in length,encoding 158 amino acid residues.The deduced protein had a molecular weight of 16 864.72 Da and a theoretical isoelectric point of 9.68.The phylogenetic tree showed that Brassica napus had the closest relationship with Brassica oleracea belonging to Brassicaceae and the farthest relationship with Oryza sativa.The analysis of the promoter region suggested that BnZAT12 may be regulated by factors such as light,abscisic acid,and methyl jasmonate.Furthermore,the BnZAT12 overexpression vector was constructed by seamless cloning.This study laid a foundation of molecular biology for further elucidating the role of BnZAT12.关键词
BnZAT12/甘蓝型油菜/生物信息学分析/载体构建Key words
BnZAT12/Brassica napus/Bioinformatics analysis/Vector construction引用本文复制引用
黄欣,周丽华,袁玉辉,钟凯,陈董龙,刘显军..甘蓝型油菜BnZAT12基因的克隆及表达载体构建[J].农业科技与技术（英文）,2025,26(1):45-54,10.基金项目
Supported by Hunan Graduate Research Innovation Project(CX20231274) （CX20231274）
Excellent Youth Project of Hunan Provincial Department of Education(22B0844) （22B0844）
Joint Fund of Hunan Province(2023JJ50083) （2023JJ50083）
Key Project of Education Reform in Hunan Province(HNJG-2022-0294)湖南省研究生科研创新项目(CX20231274) （HNJG-2022-0294）
湖南省教育厅优秀青年项目(22B0844) （22B0844）
湖南省联合基金项目(2023JJ50083) （2023JJ50083）
湖南省教育改革重点项目(HNJG-2022-0294) （HNJG-2022-0294）
